ID 83931
著者
アベ, アキコ Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
古本, 博孝 Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
ヨシダ, カナコ Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
加藤, 剛志 Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
サイジョウ, ヤスヨ Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
苛原, 稔 Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
fms-like tyrosine kinase 3 ligand
gene gun
dendritic cells
資料タイプ
学術雑誌論文
抄録
Aim : Particle-mediated transfection is known as an efficient method of nonviral
gene transfer. Flt3 ligand (FL) is a growth factor for hematopoietic progenitors ; it
promotes the growth of dendritic cells (DC). DCs are powerful antigen-presenting cells
(APCs) and show a remarkable capacity to stimulate antigen-specific T-cell responses.
In this study, we intended to investigate the suppressive effect on tumor growth by gene
gun-mediated transfer of FL in a murine model. Methods : C57BL/6J mice were injected
intradermally with MCA205 cells. DNA (pNGVL-hFLex)-coated gold particles were delivered
to themouse skin surrounding the target tumor. The expression of FL was determined
by RT-PCR. Analyses by immunohistochemistry and fluorescence-activated cell
sorter (FACS) revealed an increase in the number of DC after treatment with FL. Results :
Gene gun-mediated pNGVL-hFLex transfer significantly inhibited the growth of the
MCA205 tumor. FL transfer markedly increased the number of CD11c+ DCs in the tumor
tissue. Further, the FL-transfected mice exhibited a significantly higher number of CD80+
MHC-II cells. Conclusion : We successfully performed FL therapy using an in vivo gene
gun in order to effectively mobilize DCs in situ and induce suppressive immunity.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
58
1-2
開始ページ
39
終了ページ
45
並び順
39
発行日
2011-02
備考
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID
フルテキストファイル
言語
eng
部局
医学系
病院