ID 99853
Author
Yasui, Sonoko Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Mawatari, Kazuaki Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Morizumi, Ran Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
Furukawa, Hiroko Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Health Science, University of Kochi
Shimohata, Takaaki Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Harada, Nagakatsu Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Takahashi, Akira Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nakaya, Yutaka Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
insulin
KATP channel
ROS
oxidative stress
VSMCs
Content Type
Journal Article
Description
Both reactive oxygen species (ROS) and insulin resistance have been reported to play essential pathophysiological roles in cardiovascular diseases, such as hypertension and atherosclerosis. However, the mechanistic link between ROS and insulin resistance in the vasculature remains unclear. Recently we have shown that insulin causes membrane hyperpolarization via ATP-sensitive potassium (KATP) channel activation, which is mediated by phosphatidylinositol 3-kinase (PI3-K) in cultured vascular smooth muscle cells (VSMCs). KATP channel in the vasculature is critical in the regulation of vascular tonus. Here we examined the effects of ROS induced by hydrogen peroxide (H2O2) on insulin-induced KATP channel activities in cultured VSMCs, A10 cells. H2O2 (10 μM) increased significantly intercellular ROS in A10 cells. By using a cell-attached patch clamp experiment, 10 μMH2O2 suppressed significantly insulin-induced KATP channel activation without inhibition of insulin receptor signal transduction component including IRS and Akt in A10 cells. Furthermore 10 μMH2O2 suppressed significantly pinacidil-induced KATP channel activation in A10 cells. These data suggest that H2O2might inhibit directly KATP channel independent of insulin signaling pathway. This study may contribute to our understanding of mechanisms of insulin resistance-associated cardiovascular disease.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
59
Issue
1-2
Start Page
36
End Page
44
Sort Key
36
Published Date
2012-02
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences