アクセス数 : ?
ダウンロード数 : ?
ID 99853
著者
安井, 苑子 Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
馬渡, 一諭 Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
モリズミ, ラン Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
フルカワ, ヒロコ Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Health Science, University of Kochi
下畑, 隆明 Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
原田, 永勝 Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
髙橋, 章 Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
中屋, 豊 Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
insulin
KATP channel
ROS
oxidative stress
VSMCs
資料タイプ
学術雑誌論文
抄録
Both reactive oxygen species (ROS) and insulin resistance have been reported to play essential pathophysiological roles in cardiovascular diseases, such as hypertension and atherosclerosis. However, the mechanistic link between ROS and insulin resistance in the vasculature remains unclear. Recently we have shown that insulin causes membrane hyperpolarization via ATP-sensitive potassium (KATP) channel activation, which is mediated by phosphatidylinositol 3-kinase (PI3-K) in cultured vascular smooth muscle cells (VSMCs). KATP channel in the vasculature is critical in the regulation of vascular tonus. Here we examined the effects of ROS induced by hydrogen peroxide (H2O2) on insulin-induced KATP channel activities in cultured VSMCs, A10 cells. H2O2 (10 μM) increased significantly intercellular ROS in A10 cells. By using a cell-attached patch clamp experiment, 10 μMH2O2 suppressed significantly insulin-induced KATP channel activation without inhibition of insulin receptor signal transduction component including IRS and Akt in A10 cells. Furthermore 10 μMH2O2 suppressed significantly pinacidil-induced KATP channel activation in A10 cells. These data suggest that H2O2might inhibit directly KATP channel independent of insulin signaling pathway. This study may contribute to our understanding of mechanisms of insulin resistance-associated cardiovascular disease.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
59
1-2
開始ページ
36
終了ページ
44
並び順
36
発行日
2012-02
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系