ID 106360
Author
Yoshikawa, Kozo Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Shimada, Mitsuo Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kuwahara, Tomomi Department of Microbiology, Faculty of Medicine, Kagawa University
Hirakawa, Hideki Kazusa DNA Research Institute
Kurita, Nobuhiro Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Sato, Hirohiko Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
Utsunomiya, Tohru Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Iwata, Takashi Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Miyatani, Tomohiko Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Higashijima, Jun Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kashihara, Hideya Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Takasu, Chie Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Matsumoto, Noriko Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
Nakayama-Imaohji, Haruyuki Department of Microbiology, Faculty of Medicine, Kagawa University
Keywords
Daikenchuto
microbiome
fast stress
Content Type
Journal Article
Description
[Purpose] Diversity of gut microbiome has been recently reported to be lost in inflammatory bowel disease. We have previously reported that the Dai-kenchu-to (DKT) prevented the bacterial translocation through suppression of cytokine and apoptosis in rat’s fast stress model. The aim of this study was to evaluate the effect of DKT on maintenance of microbial diversity in rat’s intestine with inflammation. [Method] Wister rats were received the fast stress for 5 days. In DKT group, rats were administered with DKT (300 mg/kg/day) during the fast stress (DKT-group). The gut microbiomes were analyzed at before- and after- fast stress, and the effect of DKT for on microbial diversities of the gut were evaluated by the PCR-clone library method targeting the 16 S ribosomal RNA gene. [Result] In Control-group, Erysipelotrichaceae increased to 86% in after fast stress, OTU of before-fast stress was 111 and after fast stress was only 9 (changing rate : 58%). The diversity of microbiome was severely decreased. On the other hand, in DKT-group, diversity of microbiome was kept after fast stress (Lachnospiraceae : Ruminococcaceae : Coriobacteriales 54%, 22%, 5%), Operational taxonomic units of before fast stress was 52 and after fast stress was 55 (changing rate : 6%). Family Lachnospiraceae which includes butyrate-producing Clostridia (Clostridium IV and XIVa). [Conclusion] DKT prevented the reduction of diversity of microbiome in rat’s fast stress model. Our data suggested the new anti-inflammatory mechanism of DKT through gut microbiome.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
60
Issue
3-4
Start Page
221
End Page
227
Sort Key
221
Published Date
2013-08
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
University Hospital
Medical Sciences
Liberal Arts and Sciences