ID 106360
著者
吉川, 幸造 Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
島田, 光生 Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
桑原, 知巳 Department of Microbiology, Faculty of Medicine, Kagawa University
ヒラカワ, ヒデキ Kazusa DNA Research Institute
クリタ, ノブヒロ Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
サトウ, ヒロヒコ Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
ウツノミヤ, トオル Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
岩田, 貴 Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
ミヤタニ, トモヒコ Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
東島, 潤 Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
カシハラ, ヒデヤ Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
高須, 千絵 Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
マツモト, ノリコ Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
ナカヤマ イマオウジ, ハルユキ Department of Microbiology, Faculty of Medicine, Kagawa University
キーワード
Daikenchuto
microbiome
fast stress
資料タイプ
学術雑誌論文
抄録
[Purpose] Diversity of gut microbiome has been recently reported to be lost in inflammatory bowel disease. We have previously reported that the Dai-kenchu-to (DKT) prevented the bacterial translocation through suppression of cytokine and apoptosis in rat’s fast stress model. The aim of this study was to evaluate the effect of DKT on maintenance of microbial diversity in rat’s intestine with inflammation. [Method] Wister rats were received the fast stress for 5 days. In DKT group, rats were administered with DKT (300 mg/kg/day) during the fast stress (DKT-group). The gut microbiomes were analyzed at before- and after- fast stress, and the effect of DKT for on microbial diversities of the gut were evaluated by the PCR-clone library method targeting the 16 S ribosomal RNA gene. [Result] In Control-group, Erysipelotrichaceae increased to 86% in after fast stress, OTU of before-fast stress was 111 and after fast stress was only 9 (changing rate : 58%). The diversity of microbiome was severely decreased. On the other hand, in DKT-group, diversity of microbiome was kept after fast stress (Lachnospiraceae : Ruminococcaceae : Coriobacteriales 54%, 22%, 5%), Operational taxonomic units of before fast stress was 52 and after fast stress was 55 (changing rate : 6%). Family Lachnospiraceae which includes butyrate-producing Clostridia (Clostridium IV and XIVa). [Conclusion] DKT prevented the reduction of diversity of microbiome in rat’s fast stress model. Our data suggested the new anti-inflammatory mechanism of DKT through gut microbiome.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
60
3-4
開始ページ
221
終了ページ
227
並び順
221
発行日
2013-08
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
病院
医学系
教養教育院