Dual effects of FGFR inhibition in lung fibrosis
Morizumi, Shun Tokushima University Tokushima University Educator and Researcher Directory
Sato, Seidai Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Koyama, Kazuya Tokushima University
Okazaki, Hiroyasu Tokushima University
Chen, Yajuan Tokushima University
Kagawa, Kozo Tokushima University Tokushima University Educator and Researcher Directory
Ogawa, Hirohisa Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nishimura, Haruka Tokushima University
Kawano, Hiroshi Tokushima University Tokushima University Educator and Researcher Directory
Uehara, Hisanori Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Fibroblast growth factor
Thesis or Dissertation
[Rationale] Fibroblast growth factors (FGF) are major factors associated with the pathogenesis of pulmonary fibrosis. Nintedanib, a tyrosine kinase inhibitor targeting several growth factor receptors including the FGF receptor (FGFR), has been approved for the treatment of idiopathic pulmonary fibrosis (IPF). On the other hand, recent reports suggest that FGF are required for epithelial recovery. In this study, we focused on FGF signaling to both fibroblasts and alveolar epithelial cells (AECs), and examined the effect of a pan-FGFR blocker on experimental pulmonary fibrosis in mice.
[Methods] The effects of BGJ398, a pan-FGFR inhibitor, on the migration and proliferation of fibroblasts and AECs were assessed using transwell migration or 3H-thymidine incorporation assays. The expression of FGFR was analyzed using immunoblot or flow cytometry. We also investigated the effect of BGJ398 on the pulmonary fibrosis induced by bleomycin in mice.
[Results] Both lung fibroblasts and AECs expressed FGFRs. BGJ398 significantly inhibited the proliferation and migration of lung fibroblasts stimulated with FGF2. BGJ398 also reduced the proliferation of AECs in response to FGF2. Although the administration of BGJ398 ameliorated pulmonary fibrosis in bleomycin-treated mice, it increased mortality due to alveolar injury and inhibition of AEC regeneration.
[Conclusions] These data suggest that the total inhibition of FGFR signaling can suppress lung fibrosis by inhibiting fibroblast activities, although alveolar injury is simultaneously caused.
American Journal of Respiratory Cell and Molecular Biology
American Thoracic Society
本論文は，著者Shun Morizumiの学位論文として提出され，学位審査・授与の対象となっている。 論文本文は2021-04-27以降公開予定。
|DOI (Published Version)|
|URL ( Publisher's Version )|
k3430_abstract_review.pdf 212 KB
|MEXT report number||
Doctor of Medical Science