ID | 119208 |
Title Alternative | Raman Spectroscopy of Osteoblast-produced Matrix under Microvibration
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Author |
Sato, Katsuya
Tokushima University
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Minamikawa, Takeo
Tokushima University
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Yasui, Takeshi
Tokushima University
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Keywords | osteoblast
vibration stimuli
cell biomechanics
calcified matrix
Raman spectroscopy
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Content Type |
Journal Article
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Description | In this study, we used Raman spectroscopy to observe the process of osteoblast calcification under microvibration stimulation. Osteoblast cultures were prepared under three conditions: the control group was cultured statically without vibration stimulation, and the 45-Hz vibration group and 90-Hz vibration group were stimulated by microvibration at 45 Hz and 90 Hz, respectively, for 30 minutes per day with an acceleration amplitude of 0.2 G. The incubation periods were 7, 9, 11 and 14 days, and observations were conducted using Raman spectroscopy after fixation. In the 45-Hz vibration group, calcification was accelerated. This group produced a large amount of highly crystalline phosphate with large crystal size and a low proportion of hyaluronan precursor and carbonate. In the 90-Hz vibration group, differentiation into mature osteoblasts was not rapid. This group produced more non-crystalline phosphate containing more hyaluronan precursors and carbonate than the other groups. The results suggest that microvibration stimulation promotes calcification, and that the composition of the calcified area may differ depending on the vibration conditions.
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Journal Title |
Advanced Biomedical Engineering
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ISSN | 21875219
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Publisher | Japanese Society for Medical and Biological Engineering
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Volume | 13
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Start Page | 11
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End Page | 18
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Published Date | 2024
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Rights | This is an open access article distributed under the terms of the Creative Commons BY 4.0 International (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited.
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language |
eng
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departments |
Science and Technology
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