ID | 119208 |
タイトル別表記 | Raman Spectroscopy of Osteoblast-produced Matrix under Microvibration
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著者 | |
キーワード | osteoblast
vibration stimuli
cell biomechanics
calcified matrix
Raman spectroscopy
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資料タイプ |
学術雑誌論文
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抄録 | In this study, we used Raman spectroscopy to observe the process of osteoblast calcification under microvibration stimulation. Osteoblast cultures were prepared under three conditions: the control group was cultured statically without vibration stimulation, and the 45-Hz vibration group and 90-Hz vibration group were stimulated by microvibration at 45 Hz and 90 Hz, respectively, for 30 minutes per day with an acceleration amplitude of 0.2 G. The incubation periods were 7, 9, 11 and 14 days, and observations were conducted using Raman spectroscopy after fixation. In the 45-Hz vibration group, calcification was accelerated. This group produced a large amount of highly crystalline phosphate with large crystal size and a low proportion of hyaluronan precursor and carbonate. In the 90-Hz vibration group, differentiation into mature osteoblasts was not rapid. This group produced more non-crystalline phosphate containing more hyaluronan precursors and carbonate than the other groups. The results suggest that microvibration stimulation promotes calcification, and that the composition of the calcified area may differ depending on the vibration conditions.
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掲載誌名 |
Advanced Biomedical Engineering
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ISSN | 21875219
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出版者 | Japanese Society for Medical and Biological Engineering
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巻 | 13
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開始ページ | 11
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終了ページ | 18
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発行日 | 2024
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権利情報 | This is an open access article distributed under the terms of the Creative Commons BY 4.0 International (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited.
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言語 |
eng
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著者版フラグ |
出版社版
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部局 |
理工学系
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