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タイトル別表記
Raman Spectroscopy of Osteoblast-produced Matrix under Microvibration
著者
キーワード
osteoblast
vibration stimuli
cell biomechanics
calcified matrix
Raman spectroscopy
資料タイプ
学術雑誌論文
抄録
In this study, we used Raman spectroscopy to observe the process of osteoblast calcification under microvibration stimulation. Osteoblast cultures were prepared under three conditions: the control group was cultured statically without vibration stimulation, and the 45-Hz vibration group and 90-Hz vibration group were stimulated by microvibration at 45 Hz and 90 Hz, respectively, for 30 minutes per day with an acceleration amplitude of 0.2 G. The incubation periods were 7, 9, 11 and 14 days, and observations were conducted using Raman spectroscopy after fixation. In the 45-Hz vibration group, calcification was accelerated. This group produced a large amount of highly crystalline phosphate with large crystal size and a low proportion of hyaluronan precursor and carbonate. In the 90-Hz vibration group, differentiation into mature osteoblasts was not rapid. This group produced more non-crystalline phosphate containing more hyaluronan precursors and carbonate than the other groups. The results suggest that microvibration stimulation promotes calcification, and that the composition of the calcified area may differ depending on the vibration conditions.
掲載誌名
Advanced Biomedical Engineering
ISSN
21875219
出版者
Japanese Society for Medical and Biological Engineering
13
開始ページ
11
終了ページ
18
発行日
2024
権利情報
This is an open access article distributed under the terms of the Creative Commons BY 4.0 International (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
理工学系