| ID | 111037 |
| Title Alternative | Intelligent Measurement and Control of Tissue Collagen for Bone Tissue Engineering
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| Author |
Yasui, Takeshi
Tokushima University
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Sato, Katsuya
Tokushima University
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| Keywords | bone
collagen
osteoblasts
tissue engineering
second-harmonic-generation
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| Content Type |
Departmental Bulletin Paper
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| Description | Second-harmonic-generation (SHG) microscopy is a new tool for observing the collagen fiber in tissue in vivo. However, conventional SHG microscopy equipped with 100-fs pulse laser is insufficient to visualize collagen fiber produced by cultured cell with high contrast because of low nonlinear susceptibility x(2) in low-order-structured, immature collagen. To enhance the image contrast while avoiding the sample damage, one has to increase a peak power of the laser light while maintaining the same average power as the 100-fs laser. In this paper, we enhanced the sensitive of SHG microscopy by use of a 10-fs Ti:Sapphire laser and succeeded to visualize collagen fibers produced by the cultured osteoblasts in vivo.
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| Journal Title |
Bulletin of Institute of Technology and Science
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| ISSN | 21859094
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| NCID | AA12214889
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| Volume | 60
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| Start Page | 16
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| End Page | 20
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| Sort Key | 16
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| Published Date | 2015
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| Rights | この論文の著作権は徳島大学ソシオテクノサイエンス研究部にあります。
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| FullText File | |
| language |
jpn
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| TextVersion |
Publisher
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| departments |
Science and Technology
University Hospital
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