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ID 115184
Author
Goto, Yuriko Iwate Medical University
Ibi, Miho Iwate Medical University
Sato, Hirotaka Iwate Medical University
Tanaka, Junichi Showa University
Yasuhara, Rika Showa University
Fukada, Toshiyuki Showa University|Tokushima Bunri University|RIKEN Center for Integrative Medical Sciences
Mishima, Kenji Showa University
Irié, Tarou Iwate Medical University|Showa University
Keywords
Salivary gland neoplasm
Tumorigenesis
Pleomorphic adenoma gene 1
Stemness
Acinar cell
Content Type
Journal Article
Description
Objectives: Details of the histogenesis of salivary gland tumors are largely unknown. The oncogenic role of PLAG1 in the salivary gland has been demonstrated in vivo. Herein, we demonstrate the roles of PLAG1 in the acinar and ductal cells of normal human salivary glands in an attempt to clarify the early events that occur during the histogenesis of salivary gland tumors.
Methods: Normal salivary gland cells with acinar- (NS-SV-AC) and ductal- (NS-SV-DC) phenotypes were transfected with PLAG1 plasmid DNA. Subsequently, the PLAG1 overexpressed and mock cells were examined by cell proliferation, transwell migration, and salisphere formation assays. The expression levels of salivary and pluripotent stem cell markers and differentiation markers were evaluated by quantitative real-time polymerase chain reaction and immunofluorescence. Alterations in transcriptional expressions were investigated via cap analysis of gene expression with gene-enrichment and functional annotation analysis.
Results: PLAG1 promoted cell proliferation and transwell migration in the acinar and ductal cells, and markedly enhanced the stemness profiles and luminal cell-like profiles in acinar cells; the stemness profiles were partially increased in the ductal cells.
Conclusion: PLAG1 enhanced the stemness profiles in the acinar cells of normal human salivary glands in a cell type-specific manner. Thus, it may be involved in salivary gland tumorigenesis by increasing the stemness character of the normal salivary gland cells.
Journal Title
Journal of Oral Biosciences
ISSN
13490079
NCID
AA11896386
Publisher
The Japanese Association for Oral Biology|Elsevier
Volume
62
Issue
1
Start Page
99
End Page
106
Published Date
2020-01-30
Rights
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
University Hospital
Oral Sciences