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ID 115184
著者
Goto, Yuriko Iwate Medical University
Ibi, Miho Iwate Medical University
Sato, Hirotaka Iwate Medical University
Tanaka, Junichi Showa University
Yasuhara, Rika Showa University
Fukada, Toshiyuki Showa University|Tokushima Bunri University|RIKEN Center for Integrative Medical Sciences
Mishima, Kenji Showa University
Irié, Tarou Iwate Medical University|Showa University
キーワード
Salivary gland neoplasm
Tumorigenesis
Pleomorphic adenoma gene 1
Stemness
Acinar cell
資料タイプ
学術雑誌論文
抄録
Objectives: Details of the histogenesis of salivary gland tumors are largely unknown. The oncogenic role of PLAG1 in the salivary gland has been demonstrated in vivo. Herein, we demonstrate the roles of PLAG1 in the acinar and ductal cells of normal human salivary glands in an attempt to clarify the early events that occur during the histogenesis of salivary gland tumors.
Methods: Normal salivary gland cells with acinar- (NS-SV-AC) and ductal- (NS-SV-DC) phenotypes were transfected with PLAG1 plasmid DNA. Subsequently, the PLAG1 overexpressed and mock cells were examined by cell proliferation, transwell migration, and salisphere formation assays. The expression levels of salivary and pluripotent stem cell markers and differentiation markers were evaluated by quantitative real-time polymerase chain reaction and immunofluorescence. Alterations in transcriptional expressions were investigated via cap analysis of gene expression with gene-enrichment and functional annotation analysis.
Results: PLAG1 promoted cell proliferation and transwell migration in the acinar and ductal cells, and markedly enhanced the stemness profiles and luminal cell-like profiles in acinar cells; the stemness profiles were partially increased in the ductal cells.
Conclusion: PLAG1 enhanced the stemness profiles in the acinar cells of normal human salivary glands in a cell type-specific manner. Thus, it may be involved in salivary gland tumorigenesis by increasing the stemness character of the normal salivary gland cells.
掲載誌名
Journal of Oral Biosciences
ISSN
13490079
cat書誌ID
AA11896386
出版者
The Japanese Association for Oral Biology|Elsevier
62
1
開始ページ
99
終了ページ
106
発行日
2020-01-30
備考
論文本文は2021-01-30以降公開予定
権利情報
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
言語
eng
著者版フラグ
その他
部局
病院
歯学系