Yuehui, Zhang Tokushima University
Hosoki, Maki Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Oshima, Masamitsu Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Tajima, Toyoko Tokushima University KAKEN Search Researchers
Miyagi, Mayu Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Raman, Swarnalakshmi Tokushima University
Raju, Resmi National Institutes of Health
allergic contact dermatitis
MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis (n = 3) were enrolled along with additional female participants with no medical history of metal allergy (n = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
|DOI (Published Version)|
|URL ( Publisher's Version )|
biomed_11_2_277.pdf 2.9 MB