ID | 117923 |
Author |
Yuehui, Zhang
Tokushima University
Hosoki, Maki
Tokushima University
Tokushima University Educator and Researcher Directory
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Oshima, Masamitsu
Tokushima University
Tokushima University Educator and Researcher Directory
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Miyagi, Mayu
Tokushima University
Tokushima University Educator and Researcher Directory
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Raman, Swarnalakshmi
Tokushima University
Raju, Resmi
National Institutes of Health
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Keywords | allergic contact dermatitis
biomarker
metal allergy
microRNA
peripheral blood
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Content Type |
Journal Article
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Description | MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis (n = 3) were enrolled along with additional female participants with no medical history of metal allergy (n = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies.
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Journal Title |
Biomedicines
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ISSN | 22279059
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Publisher | MDPI
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Volume | 11
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Issue | 2
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Start Page | 277
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Published Date | 2023-01-19
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Rights | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
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language |
eng
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TextVersion |
Publisher
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departments |
Oral Sciences
University Hospital
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