ID | 117923 |
著者 |
Yuehui, Zhang
Tokushima University
Raman, Swarnalakshmi
Tokushima University
Raju, Resmi
National Institutes of Health
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キーワード | allergic contact dermatitis
biomarker
metal allergy
microRNA
peripheral blood
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資料タイプ |
学術雑誌論文
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抄録 | MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis (n = 3) were enrolled along with additional female participants with no medical history of metal allergy (n = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies.
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掲載誌名 |
Biomedicines
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ISSN | 22279059
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出版者 | MDPI
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巻 | 11
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号 | 2
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開始ページ | 277
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発行日 | 2023-01-19
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権利情報 | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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言語 |
eng
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著者版フラグ |
出版社版
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部局 |
歯学系
病院
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