ID | 114900 |
Author |
Yamada, Hisatsugu
Kyoto University
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Kameda, Tetsuro
Kyoto University
Kimura, Yu
Kyoto University
Imai, Hirohiko
Kyoto University
Matsuda, Tetsuya
Kyoto University
Sando, Shinsuke
The University of Tokyo
Toshimitsu, Akio
Kyoto University
Aoyama, Yasuhiro
Kyoto University
Kondo, Teruyuki
Kyoto University
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Keywords | dopamine
L-dopa
metabolic analysis
neurotransmitters
stable isotope enrichment
triple-resonance NMR
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Content Type |
Journal Article
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Description | In an attempt to monitor μM-level trace constituents, we applied here 1H-{13C-15N} triple-resonance nuclear magnetic resonance (NMR) to 13C/15N-enriched L-Dopa as the inevitable precursor of the neurotransmitter dopamine in the brain. The perfect selectivity (to render endogenous components silent) and μM-level sensitivity (700 MHz spectrometer equipped with a cryogenic probe) of triple-resonance allowed the unambiguous and quantitative metabolic and pharmacokinetic analyses of administered L-Dopa/dopamine in the brain and liver of mice. The level of dopamine generated in the brain (within the range 7–76 μM, which covers the typical stimulated level of ~30 μM) could be clearly monitored ex vivo, but was slightly short of the detection limit of a 7T MR machine for small animals. This work suggests that μM-level trace constituents are potential targets of ex vivo monitoring as long as they contain N atom(s) and their appropriate 13C/15N-enrichment is synthetically accessible.
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Journal Title |
ChemistryOpen
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ISSN | 21911363
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Publisher | Wiley-VCH Verlag GmbH & Co. KGaA
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Volume | 5
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Issue | 2
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Start Page | 125
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End Page | 128
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Published Date | 2015-12-30
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Rights | © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License(https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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DOI (Published Version) | |
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language |
eng
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TextVersion |
Publisher
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departments |
Bioscience and Bioindustry
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