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ID 113586
Title Alternative
Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via crosstalk between integrin β1 and CD63
Ameloblastin regulates osteogenic differentiation
Author
Iizuka, Shinji Hiroshima University
Yoshida, Maki Hiroshima University
Yoshiko, Yuji Hiroshima University
Uchida, Takashi Hiroshima University
Ogawa, Ikuko Hiroshima University
Miyauchi, Mutsumi Hiroshima University
Takata, Takashi Hiroshima University
Content Type
Journal Article
Description
Ameloblastin, the most abundant non-amelogenin enamel matrix protein, plays a role in ameloblast differentiation. Here we found that ameloblastin was expressed in osteosarcoma cells; to explore the potential functions of ameloblastin in osteoblasts, we investigated whether this protein is involved in osteogenic differentiation and bone formation on the premise that CD63, a member of the transmembrane-4 glycoprotein superfamily, interacts with integrins in the presence of ameloblastin. Ameloblastin bound to CD63 and promoted CD63 binding to integrin β1. The interaction between CD63 and integrin β1 induced Src kinase inactivation via the binding of CD63 to Src. The reduction of Src activity and osteogenic differentiation mediated by ameloblastin was abrogated by treatment with anti-CD63 antibody and overexpression of constitutive active Src, respectively. Moreover, amelobastin upregulated the formation of stress-fibre and focal adhesions and downregulated cell migration in association with RhoA regulation via Src activity. Therefore, our results suggest that ameloblastin is expressed in osteoblasts and functions as a promoting factor for osteogenic differentiation via a novel pathway through the interaction between CD63 and integrin β1.
Journal Title
Molecular and Cellular Biology
ISSN
02707306
10985549
NCID
AA10620925
Publisher
American Society for Microbiology
Volume
31
Issue
4
Start Page
783
End Page
792
Published Date
2011-01-26
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Oral Sciences
Medical Sciences