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ID 113586
タイトル別表記
Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via crosstalk between integrin β1 and CD63
Ameloblastin regulates osteogenic differentiation
著者
Iizuka, Shinji Hiroshima University
Yoshida, Maki Hiroshima University
Yoshiko, Yuji Hiroshima University
Uchida, Takashi Hiroshima University
Ogawa, Ikuko Hiroshima University
宮内, 睦美 Hiroshima University
高田, 隆 Hiroshima University
資料タイプ
学術雑誌論文
抄録
Ameloblastin, the most abundant non-amelogenin enamel matrix protein, plays a role in ameloblast differentiation. Here we found that ameloblastin was expressed in osteosarcoma cells; to explore the potential functions of ameloblastin in osteoblasts, we investigated whether this protein is involved in osteogenic differentiation and bone formation on the premise that CD63, a member of the transmembrane-4 glycoprotein superfamily, interacts with integrins in the presence of ameloblastin. Ameloblastin bound to CD63 and promoted CD63 binding to integrin β1. The interaction between CD63 and integrin β1 induced Src kinase inactivation via the binding of CD63 to Src. The reduction of Src activity and osteogenic differentiation mediated by ameloblastin was abrogated by treatment with anti-CD63 antibody and overexpression of constitutive active Src, respectively. Moreover, amelobastin upregulated the formation of stress-fibre and focal adhesions and downregulated cell migration in association with RhoA regulation via Src activity. Therefore, our results suggest that ameloblastin is expressed in osteoblasts and functions as a promoting factor for osteogenic differentiation via a novel pathway through the interaction between CD63 and integrin β1.
掲載誌名
Molecular and Cellular Biology
ISSN
02707306
10985549
cat書誌ID
AA10620925
出版者
American Society for Microbiology
31
4
開始ページ
783
終了ページ
792
発行日
2011-01-26
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
歯学系
医学系