ID | 119259 |
Title Alternative | Vibrio vulnificusから産生される細胞外小胞の特徴について
Characterization of OMVs by V. vulnificus
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Author |
Higashiyama, Ryo
Tokushima University
Kanda, Yuna
Tokushima University
Fukushima, Shiho
Tokushima University
Yamazaki, Kohei
Kitasato University
Uebanso, Takashi
Tokushima University
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Mawatari, Kazuaki
Tokushima University
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Kashimoto, Takashige
Kitasato University
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Keywords | Infection
Outer Membrane Vesicle
Pathogenicity
Serine Protease
Vibrio Vulnificus
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Content Type |
Thesis or Dissertation
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Description | Vibrio vulnificus (V. vulnificus) is a halophilic gram-negative bacterium that inhabits coastal warm water and induce severe diseases such as primary septicemia. To investigate the mechanisms of rapid bacterial translocation on intestinal infection, we focused on outer membrane vesicles (OMVs), which are extracellular vesicles produced by Gram-negative bacteria and deliver virulence factors. However, there are very few studies on the pathogenicity or contents of V. vulnificus OMVs (Vv-OMVs). In this study, we investigated the effects of Vv-OMVs on host cells. Epithelial cells INT407 were stimulated with purified OMVs and morphological alterations and levels of lactate dehydrogenase (LDH) release were observed. In cells treated with OMVs, cell detachment without LDH release was observed, which exhibited different characteristics from cytotoxic cell detachment observed in V. vulnificus infection. Interestingly, OMVs from a Vibrio Vulnificus Hemolysin (VVH) and Multifunctional-autoprocessing repeats-in -toxin (MARTX) double-deletion mutant strain also caused cell detachment without LDH release. Our results suggested that the proteolytic function of a serine protease contained in Vv-OMVs may contribute to pathogenicity of V. vulnificus by assisting bacterial translocation. This study reveals a new pathogenic mechanism during V. vulnificus infections.
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Journal Title |
The Journal of Medical Investigation
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ISSN | 13496867
13431420
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NCID | AA11166929
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Publisher | Tokushima University Faculty of Medicine
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Volume | 71
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Issue | 1-2
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Start Page | 102
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End Page | 112
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Sort Key | 102
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Published Date | 2024-02
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Ryo Higashiyamaの学位論文として提出され,学位審査・授与の対象となっている。 |
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3774号
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Diploma Number | 甲栄第302号
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Granted Date | 2024-02-22
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Degree Name |
Doctor of Nutritional Science
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Grantor |
Tokushima University
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departments |
Medical Sciences
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