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ID 119259
Title Alternative
Vibrio vulnificusから産生される細胞外小胞の特徴について
Characterization of OMVs by V. vulnificus
Author
Higashiyama, Ryo Tokushima University
Kanda, Yuna Tokushima University
Shimohata, Takaaki Tokushima University|Fukui Prefectural University KAKEN Search Researchers
Fukushima, Shiho Tokushima University
Yamazaki, Kohei Kitasato University
Kashimoto, Takashige Kitasato University
Keywords
Infection
Outer Membrane Vesicle
Pathogenicity
Serine Protease
Vibrio Vulnificus
Content Type
Thesis or Dissertation
Description
Vibrio vulnificus (V. vulnificus) is a halophilic gram-negative bacterium that inhabits coastal warm water and induce severe diseases such as primary septicemia. To investigate the mechanisms of rapid bacterial translocation on intestinal infection, we focused on outer membrane vesicles (OMVs), which are extracellular vesicles produced by Gram-negative bacteria and deliver virulence factors. However, there are very few studies on the pathogenicity or contents of V. vulnificus OMVs (Vv-OMVs). In this study, we investigated the effects of Vv-OMVs on host cells. Epithelial cells INT407 were stimulated with purified OMVs and morphological alterations and levels of lactate dehydrogenase (LDH) release were observed. In cells treated with OMVs, cell detachment without LDH release was observed, which exhibited different characteristics from cytotoxic cell detachment observed in V. vulnificus infection. Interestingly, OMVs from a Vibrio Vulnificus Hemolysin (VVH) and Multifunctional-autoprocessing repeats-in -toxin (MARTX) double-deletion mutant strain also caused cell detachment without LDH release. Our results suggested that the proteolytic function of a serine protease contained in Vv-OMVs may contribute to pathogenicity of V. vulnificus by assisting bacterial translocation. This study reveals a new pathogenic mechanism during V. vulnificus infections.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
Publisher
Tokushima University Faculty of Medicine
Volume
71
Issue
1-2
Start Page
102
End Page
112
Sort Key
102
Published Date
2024-02
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Ryo Higashiyamaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3774号
Diploma Number
甲栄第302号
Granted Date
2024-02-22
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences