ID | 119259 |
タイトル別表記 | Vibrio vulnificusから産生される細胞外小胞の特徴について
Characterization of OMVs by V. vulnificus
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著者 |
東山, 諒
徳島大学大学院医科栄養学研究科(医科栄養学専攻)
Kanda, Yuna
Tokushima University
Fukushima, Shiho
Tokushima University
Yamazaki, Kohei
Kitasato University
Kashimoto, Takashige
Kitasato University
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キーワード | Infection
Outer Membrane Vesicle
Pathogenicity
Serine Protease
Vibrio Vulnificus
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資料タイプ |
学位論文
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抄録 | Vibrio vulnificus (V. vulnificus) is a halophilic gram-negative bacterium that inhabits coastal warm water and induce severe diseases such as primary septicemia. To investigate the mechanisms of rapid bacterial translocation on intestinal infection, we focused on outer membrane vesicles (OMVs), which are extracellular vesicles produced by Gram-negative bacteria and deliver virulence factors. However, there are very few studies on the pathogenicity or contents of V. vulnificus OMVs (Vv-OMVs). In this study, we investigated the effects of Vv-OMVs on host cells. Epithelial cells INT407 were stimulated with purified OMVs and morphological alterations and levels of lactate dehydrogenase (LDH) release were observed. In cells treated with OMVs, cell detachment without LDH release was observed, which exhibited different characteristics from cytotoxic cell detachment observed in V. vulnificus infection. Interestingly, OMVs from a Vibrio Vulnificus Hemolysin (VVH) and Multifunctional-autoprocessing repeats-in -toxin (MARTX) double-deletion mutant strain also caused cell detachment without LDH release. Our results suggested that the proteolytic function of a serine protease contained in Vv-OMVs may contribute to pathogenicity of V. vulnificus by assisting bacterial translocation. This study reveals a new pathogenic mechanism during V. vulnificus infections.
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掲載誌名 |
The Journal of Medical Investigation
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ISSN | 13496867
13431420
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cat書誌ID | AA11166929
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出版者 | Tokushima University Faculty of Medicine
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巻 | 71
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号 | 1-2
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開始ページ | 102
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終了ページ | 112
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並び順 | 102
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発行日 | 2024-02
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備考 | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Ryo Higashiyamaの学位論文として提出され,学位審査・授与の対象となっている。 |
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第3774号
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学位記番号 | 甲栄第302号
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学位授与年月日 | 2024-02-22
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学位名 |
博士(栄養学)
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学位授与機関 |
徳島大学
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部局 |
医学系
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