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ID 113367
Title Alternative
成長期における食餌性リンによるα-klotho発現制御
Author
Fukuda-Tatano, Shiori University of Tokushima|The University of Shimane
Yamamoto, Hironori University of Tokushima|Jin-ai University|University of Fukui KAKEN Search Researchers
Nakahashi, Otoki University of Tokushima|Tokushima Bunri University
Yoshikawa, Ryouhei University of Tokushima
Hayashi, Mayu University of Tokushima
Kishimoto, Maki University of Tokushima
Imi, Yukiko Konan Women’s University
Keywords
Dietary phosphate
Growth periods
α-Klotho
FGF23
Vitamin D metabolism
Kidney
Content Type
Thesis or Dissertation
Description
Inorganic phosphate (Pi) is an essential nutrient for maintaining various biological functions, particularly during growth periods. Excess intake of dietary Pi increases the secretion of fibroblast growth factor 23 (FGF23) and parathyroid hormone (PTH) to maintain plasma Pi levels. FGF23 is a potent phosphaturic factor that binds to the α-klotho/FGFR complex in the kidney to promote excretion of Pi into the urine. In addition, excess intake of dietary Pi decreases renal α-klotho expression. Down-regulation or lack of α-klotho induces a premature aging-like phenotype, resulting from hyperphosphatemia, and leading to conditions such as ectopic calcification and osteoporosis. However, it remains unclear what effects dietary Pi has on α-klotho expression at different life stages, especially during growth periods. To investigate this, we used C57BL/6J mice in two life stages during growing period. Weaned (3 weeks old) and periadolescent (7 weeks old) were randomly divided into seven experimental groups and fed with 0.02, 0.3, 0.6, 0.9, 1.2, 1.5, or 1.8% Pi diets for 7 days. As a result, elevated plasma Pi and FGF23 levels and decreased renal α-klotho expression were observed in weaned mice fed with a high Pi diet. In addition, a high Pi diet clearly induced renal calcification in the weaned mice. However, in the periadolescent group, renal calcification was not observed, even in the 1.8% Pi diet group. The present study indicates that a high Pi diet in weaned mice has much greater adverse effects on renal α-klotho expression and pathogenesis of renal calcification compared with periadolescent mice.
Journal Title
Calcified Tissue International
ISSN
0171967X
14320827
NCID
AA00141085
Publisher
Springer Nature
Volume
104
Issue
6
Start Page
667
End Page
678
Published Date
2019-01-23
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Shiori Fukuda-Tatanoの学位論文として提出され,学位審査・授与の対象となっている。
This is a post-peer-review, pre-copyedit version of an article published in Calcified Tissue International.
The final authenticated version is available online at: https://doi.org/10.1007/s00223-019-00525-0
Rights
© 2019, Springer Nature
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3278号
Diploma Number
甲栄第267号
Granted Date
2019-03-22
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences