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ID 114242
Title Alternative
ストレプトゾトシン誘導性糖尿病ラットにおける腎Cyp24a1遺伝子発現上昇と血漿1,25-ジヒドロキシビタミンD濃度低下との関連
Author
Tajiri, Mari University of Tokushima
Nakahashi, Otoki University of Tokushima|Tokushima Bunri University
Kagawa, Tomohiro University of Tokushima
Iwano, Masayuki University of Fukui
Yamamoto, Hironori University of Tokushima|University of Fukui|Jin-ai University KAKEN Search Researchers
Keywords
vitamin D
CYP24A1
CYP24A1-SV
diabetes
streptozotocin
Content Type
Thesis or Dissertation
Description
Decreases in plasma vitamin D concentrations have been reported in diabetes, although the mechanism involved in this decrease is unclear. Here, we investigated the association between Cyp24a1, a vitamin D catabolic enzyme, and abnormalities in vitamin D metabolism in streptozotocin-induced diabetes rats, an animal model of type 1 diabetes. Plasma 1,25-dihydroxyvitamin D [1,25(OH)2D] levels were significantly lower in streptozotocin-induced diabetes rats and renal Cyp24a1 mRNA expression levels were increased. Western blotting analysis of streptozotocin-induced diabetes rats kidney tissues with anti-CYP24A1 antibody showed a strong signal around 40 kDa, which differs from the predicted 50–55 kDa molecular weight for full-length Cyp24a1 and could represent the Cyp24a1-splicing variant that lacks exons 1 and 2. We observed high levels of renal Cyp24a1-splicing variant mRNA expression in streptozotocin-induced diabetes rats. We also confirmed transcriptional up-regulation of endogenous Cyp24a1 mRNA expression through glucocorticoid receptors by glucocorticoid in opossum kidney proximal cells. Taken together, our results indicated that high Cyp24a1 expression levels may play a role in the decrease of plasma 1,25(OH)2D levels in streptozotocin-induced diabetes rats. High plasma corticosterone levels in diabetes may affect transcriptional regulation to promote increases in Cyp24a1 expression.
Journal Title
Journal of Clinical Biochemistry and Nutrition
ISSN
09120009
18805086
NCID
AA10710201
AA12091064
Publisher
Society for Free Radical Research Japan
Volume
66
Issue
1
Start Page
49
End Page
56
Published Date
2020-01-01
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Mari Tajiriの学位論文として提出され,学位審査・授与の対象となっている。
Rights
© 2020 Society for Free Radical Research Japan
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3340号
Diploma Number
甲栄第268号
Granted Date
2019-11-28
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences