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ID 114242
タイトル別表記
ストレプトゾトシン誘導性糖尿病ラットにおける腎Cyp24a1遺伝子発現上昇と血漿1,25-ジヒドロキシビタミンD濃度低下との関連
著者
田尻, 真梨 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Nakahashi, Otoki University of Tokushima|Tokushima Bunri University
香川, 知博 University of Tokushima
Iwano, Masayuki University of Fukui
山本, 浩範 University of Tokushima|University of Fukui|Jin-ai University KAKEN研究者をさがす
キーワード
vitamin D
CYP24A1
CYP24A1-SV
diabetes
streptozotocin
資料タイプ
学位論文
抄録
Decreases in plasma vitamin D concentrations have been reported in diabetes, although the mechanism involved in this decrease is unclear. Here, we investigated the association between Cyp24a1, a vitamin D catabolic enzyme, and abnormalities in vitamin D metabolism in streptozotocin-induced diabetes rats, an animal model of type 1 diabetes. Plasma 1,25-dihydroxyvitamin D [1,25(OH)2D] levels were significantly lower in streptozotocin-induced diabetes rats and renal Cyp24a1 mRNA expression levels were increased. Western blotting analysis of streptozotocin-induced diabetes rats kidney tissues with anti-CYP24A1 antibody showed a strong signal around 40 kDa, which differs from the predicted 50–55 kDa molecular weight for full-length Cyp24a1 and could represent the Cyp24a1-splicing variant that lacks exons 1 and 2. We observed high levels of renal Cyp24a1-splicing variant mRNA expression in streptozotocin-induced diabetes rats. We also confirmed transcriptional up-regulation of endogenous Cyp24a1 mRNA expression through glucocorticoid receptors by glucocorticoid in opossum kidney proximal cells. Taken together, our results indicated that high Cyp24a1 expression levels may play a role in the decrease of plasma 1,25(OH)2D levels in streptozotocin-induced diabetes rats. High plasma corticosterone levels in diabetes may affect transcriptional regulation to promote increases in Cyp24a1 expression.
掲載誌名
Journal of Clinical Biochemistry and Nutrition
ISSN
09120009
18805086
cat書誌ID
AA10710201
AA12091064
出版者
Society for Free Radical Research Japan
66
1
開始ページ
49
終了ページ
56
発行日
2020-01-01
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Mari Tajiriの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2020 Society for Free Radical Research Japan
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3340号
学位記番号
甲栄第268号
学位授与年月日
2019-11-28
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
病院
医学系