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ID 115358
Title Alternative
Secretion dynamics of cytotoxic granules in the cytotoxicity of the human NK-like cultured cells KHYG-1
KHYG‐1における細胞傷害性と細胞傷害性顆粒の変化
Author
Miwa, Utaka Hyogo Prefectural Tamba Medical Center
Sato, Mizuki Seikei-kai Chiba Medical Center
Sone, Atsumi Tokushima University
Keywords
NK cell
KHYG‐1
cytotoxicity
granzyme B
IL‐2
Content Type
Journal Article
Description
Three major therapies, “surgery”, “chemotherapy”, and “radiotherapy”, have been used to treat cancer. Recently, “immunotherapy” has attracted attention as the fourth treatment. We previously performed fundamental studies using NK cells, one cell type that has attracted attention in immunotherapy, and revealed that the surface CD56 antigen on the KHYG‐1 human NK cell-like cultured cells as a substitute for human NK cells can be used as an evaluation index of NK cell activity as in human NK cells. We also reported that the cytotoxicity of KHYG‐1increases by IL‐2 stimulation. In this study, we improved the conventional cytotoxicity measurement method to evaluate the effects of a small amount of activator on NK cells. As a result, the cytotoxicity rate and measurement sensitivity at low-concentration IL‐2 stimulation were increased, and it became possible to evaluate the effects of a smaller amount of the activator. In the dynamic observation of KHYG‐1 intracellular granules, it was possible to observe in real time how the target cells were damaged after the influx of granules. Furthermore, the relationship between the activation of KHYG‐1and the change in the intracellular expression level of granzyme B by IL‐2stimulation was clarified, and future research tasks were shown.
Journal Title
Shikoku Acta Medica
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
76
Issue
3-4
Start Page
137
End Page
142
Sort Key
137
Published Date
2020-08-25
EDB ID
FullText File
language
jpn
TextVersion
Publisher
departments
Medical Sciences
Pharmaceutical Sciences