Secretion dynamics of cytotoxic granules in the cytotoxicity of the human NK-like cultured cells KHYG-1
三輪, 詩佳 兵庫県立丹波医療センター
佐藤, 瑞樹 誠馨会千葉メディカルセンター
曽根, 淳美 徳島大学
Three major therapies, “surgery”, “chemotherapy”, and “radiotherapy”, have been used to treat cancer. Recently, “immunotherapy” has attracted attention as the fourth treatment. We previously performed fundamental studies using NK cells, one cell type that has attracted attention in immunotherapy, and revealed that the surface CD５６ antigen on the KHYG‐１ human NK cell-like cultured cells as a substitute for human NK cells can be used as an evaluation index of NK cell activity as in human NK cells. We also reported that the cytotoxicity of KHYG‐１increases by IL‐２ stimulation. In this study, we improved the conventional cytotoxicity measurement method to evaluate the effects of a small amount of activator on NK cells. As a result, the cytotoxicity rate and measurement sensitivity at low-concentration IL‐２ stimulation were increased, and it became possible to evaluate the effects of a smaller amount of the activator. In the dynamic observation of KHYG‐１ intracellular granules, it was possible to observe in real time how the target cells were damaged after the influx of granules. Furthermore, the relationship between the activation of KHYG‐１and the change in the intracellular expression level of granzyme B by IL‐２stimulation was clarified, and future research tasks were shown.
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