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ID 115358
タイトル別表記
Secretion dynamics of cytotoxic granules in the cytotoxicity of the human NK-like cultured cells KHYG-1
KHYG‐1における細胞傷害性と細胞傷害性顆粒の変化
著者
三輪, 詩佳 兵庫県立丹波医療センター
佐藤, 瑞樹 誠馨会千葉メディカルセンター
曽根, 淳美 徳島大学
キーワード
NK cell
KHYG‐1
cytotoxicity
granzyme B
IL‐2
資料タイプ
学術雑誌論文
抄録
Three major therapies, “surgery”, “chemotherapy”, and “radiotherapy”, have been used to treat cancer. Recently, “immunotherapy” has attracted attention as the fourth treatment. We previously performed fundamental studies using NK cells, one cell type that has attracted attention in immunotherapy, and revealed that the surface CD56 antigen on the KHYG‐1 human NK cell-like cultured cells as a substitute for human NK cells can be used as an evaluation index of NK cell activity as in human NK cells. We also reported that the cytotoxicity of KHYG‐1increases by IL‐2 stimulation. In this study, we improved the conventional cytotoxicity measurement method to evaluate the effects of a small amount of activator on NK cells. As a result, the cytotoxicity rate and measurement sensitivity at low-concentration IL‐2 stimulation were increased, and it became possible to evaluate the effects of a smaller amount of the activator. In the dynamic observation of KHYG‐1 intracellular granules, it was possible to observe in real time how the target cells were damaged after the influx of granules. Furthermore, the relationship between the activation of KHYG‐1and the change in the intracellular expression level of granzyme B by IL‐2stimulation was clarified, and future research tasks were shown.
掲載誌名
四国医学雑誌
ISSN
00373699
cat書誌ID
AN00102041
出版者
徳島医学会
76
3-4
開始ページ
137
終了ページ
142
並び順
137
発行日
2020-08-25
EDB ID
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
医学系
薬学系