ID 113270
Title Alternative
子宮内膜症モデルマウスにおいてアクチビンの腹腔内投与は子宮内膜症様病変の増殖を促進する
Activin A promotes endometriotic lesions
Author
Kasai, Kana Tokushima University
Kadota, Yuri Tokushima University
Erdenebayar, Otgontsetseg Tokushima University
Yoshida, Kanako Tokushima University
Keywords
Endometriosis
Activin A
Mouse model
Inflammatory cytokine
Content Type
Thesis or Dissertation
Description
PURPOSE : This study aimed to investigate the effect of intraperitoneal administration of activin on the occurrence of endometriosis using a mouse model of endometriosis. METHODS : A mouse model of endometriosis was prepared by intraperitoneally administering endometrial tissue and blood collected from donor mice to C57BL/6J 7-8- week-old recipient mice. A total of 400 μg of activin A was intraperitoneally administered to model mice in the activin group for 5 days. Intraperitoneal endometriotic lesions were confirmed macroscopically and IL-6 and TNF-α levels in washed ascites were measured by ELISA. RESULTS : Endometriotic lesions were observed in all mice. In the activin group, the maximum diameter of endometriotic lesions was significantly larger than that in control group (4.7±1.3 vs 2.9±0.9 mm, p<0.01). The total area of the lesion was also significantly higher in the activin group than in the control group (21.1±9.9 vs 8.8±5.4 mm2, p<0.01). Furthermore, IL-6 and TNF-α levels in ascites were significantly higher in the activin group than in the control group (IL-6 : 85.8±15.3 vs 75.1±19.3 pg/ml, p<0.05 ; TNF-α : 629.8±15.4 vs 605.9±11.4 pg/ml, p<0.05). CONCLUSION : Activin promotes occurrence of endometriosis. Inflammatory cytokines are also elevated by activin administration,suggesting that they may contribute to progression of endometriosis
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA12022913
AA11166929
Publisher
Tokushima University Faculty of Medicine
Volume
66
Issue
1-2
Start Page
123
End Page
127
Sort Key
123
Published Date
2019-02
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kana Kasaiの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3253号
Diploma Number
甲医第1402号
Granted Date
2019-02-06
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences
University Hospital