ID 109578
Title Alternative
歯原性上皮細胞におけるSp6タンパク質によるRock1遺伝子プロモーター活性の制御
Sp6 regulates Rock1
Author
Yanuarysuka, Ryna Dwi the University of Tokushima
Adiningrat, Arya the University of Tokushima
Tanimura, Ayako the University of Tokushima KAKEN Search Researchers
Hagita, Hiroko the University of Tokushima
Keywords
amelogenesis
dental epithelial cells
Rock1
Sp6
transcription start site
Content Type
Thesis or Dissertation
Description
Sp6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. However, the underlying molecular mechanisms remain unclear. We have previously identified one of the Sp6 downstream genes, Rock1, which is involved in ameloblast polarization. In this study, we investigated the transcriptional regulatory mechanisms of Rock1 by Sp6. First, we identified the transcription start sites (TSS) and cloned the 5'- flanking region of Rock1. Serial deletion analyses identified a critical region for Rock1 promoter activity within the 249-bp upstream region of TSS, and chromatin immunoprecipitation assays revealed Sp6-binding to this region. Subsequent transient transfection experiments showed that Rock1 promoter activity is enhanced by Sp6, but reduced by Sp1. Treatment of dental epithelial cells with the GC-selective DNA binding inhibitor, mithramycin A, affected Rock1 promoter activity in loss of enhancement by Sp6, but not repression by Sp1. Further site-directed mutagenesis indicated that the region from -206 to -150 contains responsive elements for Sp6. Taken together, we conclude that Sp6 positively regulates Rock1 transcription by direct binding to the Rock1 promoter region from -206 to -150, which functionally distinct from Sp1.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
61
Issue
3-4
Start Page
306
End Page
317
Sort Key
306
Published Date
2014-08
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201409191004.pdf
審査要旨 : LID201409191005.pdf
論文本文 : LID201503101001.pdf
本論文は,著者Ryna Dwi Yanuaryskaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2756号
Diploma Number
甲口第385号
Granted Date
2014-09-10
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences