ID 109578
タイトル別表記
歯原性上皮細胞におけるSp6タンパク質によるRock1遺伝子プロモーター活性の制御
Sp6 regulates Rock1
著者
ヤヌアリスカ, リナ ドゥイ 徳島大学大学院口腔科学教育部(口腔科学専攻)
Adiningrat, Arya the University of Tokushima
谷村, 綾子 the University of Tokushima KAKEN研究者をさがす
ハギタ, ヒロコ the University of Tokushima
キーワード
amelogenesis
dental epithelial cells
Rock1
Sp6
transcription start site
資料タイプ
学位論文
抄録
Sp6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. However, the underlying molecular mechanisms remain unclear. We have previously identified one of the Sp6 downstream genes, Rock1, which is involved in ameloblast polarization. In this study, we investigated the transcriptional regulatory mechanisms of Rock1 by Sp6. First, we identified the transcription start sites (TSS) and cloned the 5'- flanking region of Rock1. Serial deletion analyses identified a critical region for Rock1 promoter activity within the 249-bp upstream region of TSS, and chromatin immunoprecipitation assays revealed Sp6-binding to this region. Subsequent transient transfection experiments showed that Rock1 promoter activity is enhanced by Sp6, but reduced by Sp1. Treatment of dental epithelial cells with the GC-selective DNA binding inhibitor, mithramycin A, affected Rock1 promoter activity in loss of enhancement by Sp6, but not repression by Sp1. Further site-directed mutagenesis indicated that the region from -206 to -150 contains responsive elements for Sp6. Taken together, we conclude that Sp6 positively regulates Rock1 transcription by direct binding to the Rock1 promoter region from -206 to -150, which functionally distinct from Sp1.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
61
3-4
開始ページ
306
終了ページ
317
並び順
306
発行日
2014-08
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201409191004.pdf
審査要旨 : LID201409191005.pdf
論文本文 : LID201503101001.pdf
本論文は,著者Ryna Dwi Yanuaryskaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2756号
学位記番号
甲口第385号
学位授与年月日
2014-09-10
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系