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ID 115020
Author
Fujimura, Takanori Nara Medical University
Fujimoto, Takashi Nara Medical University
Itaya-Hironaka, Asako Nara Medical University
Miyaoka, Tomoko Nara Medical University
Yoshimoto, Kiyomi Nara Medical University
Yamauchi, Akiyo Nara Medical University
Sakuramoto-Tsuchida, Sumiyo Nara Medical University
Kondo, Saori Nara Medical University
Takeda, Maiko Nara Medical University
Tsujinaka, Hiroki Nara Medical University
Tanaka, Yasuhito Nara Medical University
Takasawa, Shin Nara Medical University
Keywords
Regenerating gene
Interleukin-6
Sjögren׳s syndrome
Janus kinase
Signal transducer and activator of transcription
Content Type
Journal Article
Description
The regenerating gene, Reg, was originally isolated from a rat regenerating islet cDNA library, and its human homolog was named REG Iα. Recently, we reported that REG Iα mRNA as well as its product were overexpressed in ductal epithelial cells in the minor salivary glands of Sjögren׳s syndrome (SS) patients. This study was undertaken to elucidate the role of cytokines and the subsequent intracellular mechanism for induction of REG Iα in the salivary glands of SS patients. We prepared a reporter plasmid containing REG Iα promoter (−1190/+26) upstream of a luciferase reporter gene. The promoter plasmid was introduced by lipofection into human NS-SV-DC and rat A5 salivary ductal cells. The cells were treated with interleukin (IL)-6, IL-8, and a combination of the two. Thereafter transcriptional activity of REG Iα was measured by luciferase assay. We found that IL-6 stimulation, but not IL-8, significantly enhanced the REG Iα promoter activity in salivary ductal cells. Deletion analysis revealed that the region of −141 to −117 of the REG Iα gene was responsible for the promoter activation by IL-6, which contains a consensus sequence for signal transduction and activation of transcription (STAT). The introduction of siRNA for human STAT3 abolished IL-6-induced REG Iα transcription. These results showed that IL-6 stimulation induced REG Iα transcription through STAT3 activation and binding to the consensus sequence of REG Iα promoter in salivary ductal cells. This IL-6/STAT dependent REG Iα induction might play a role in the pathogenesis of SS.
Journal Title
Biochemistry and Biophysics Reports
ISSN
24055808
Publisher
Elsevier
Volume
2
Start Page
69
End Page
74
Published Date
2015-05-30
Rights
© 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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DOI (Published Version)
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language
eng
TextVersion
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departments
Oral Sciences