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ID 115020
著者
Fujimura, Takanori Nara Medical University
Fujimoto, Takashi Nara Medical University
Itaya-Hironaka, Asako Nara Medical University
Miyaoka, Tomoko Nara Medical University
Yoshimoto, Kiyomi Nara Medical University
Yamauchi, Akiyo Nara Medical University
Sakuramoto-Tsuchida, Sumiyo Nara Medical University
Kondo, Saori Nara Medical University
Takeda, Maiko Nara Medical University
Tsujinaka, Hiroki Nara Medical University
Tanaka, Yasuhito Nara Medical University
Takasawa, Shin Nara Medical University
キーワード
Regenerating gene
Interleukin-6
Sjögren׳s syndrome
Janus kinase
Signal transducer and activator of transcription
資料タイプ
学術雑誌論文
抄録
The regenerating gene, Reg, was originally isolated from a rat regenerating islet cDNA library, and its human homolog was named REG Iα. Recently, we reported that REG Iα mRNA as well as its product were overexpressed in ductal epithelial cells in the minor salivary glands of Sjögren׳s syndrome (SS) patients. This study was undertaken to elucidate the role of cytokines and the subsequent intracellular mechanism for induction of REG Iα in the salivary glands of SS patients. We prepared a reporter plasmid containing REG Iα promoter (−1190/+26) upstream of a luciferase reporter gene. The promoter plasmid was introduced by lipofection into human NS-SV-DC and rat A5 salivary ductal cells. The cells were treated with interleukin (IL)-6, IL-8, and a combination of the two. Thereafter transcriptional activity of REG Iα was measured by luciferase assay. We found that IL-6 stimulation, but not IL-8, significantly enhanced the REG Iα promoter activity in salivary ductal cells. Deletion analysis revealed that the region of −141 to −117 of the REG Iα gene was responsible for the promoter activation by IL-6, which contains a consensus sequence for signal transduction and activation of transcription (STAT). The introduction of siRNA for human STAT3 abolished IL-6-induced REG Iα transcription. These results showed that IL-6 stimulation induced REG Iα transcription through STAT3 activation and binding to the consensus sequence of REG Iα promoter in salivary ductal cells. This IL-6/STAT dependent REG Iα induction might play a role in the pathogenesis of SS.
掲載誌名
Biochemistry and Biophysics Reports
ISSN
24055808
出版者
Elsevier
2
開始ページ
69
終了ページ
74
発行日
2015-05-30
権利情報
© 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系