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ID 114636
Title Alternative
Evaluation of NK cell function using human NK-like cultured cell, KHYG-1
CD56抗原を指標としたNK様培養細胞の活性評価
Author
Sato, Mizuki Seikei−kai Chiba Medical Center
Sone, Atsumi Tokushima University
Keywords
NK cell
KHYG‐1
CD56 antigen
IL‐2
Content Type
Journal Article
Description
NK cells play an important role in the elimination of viral infection and tumors. In recent years, immune cell therapy using activated NK cells has attracted attention, and the search for activators and evaluation of NK cell activity have become important. We previously reported a positive correlation between CD56antigen expression, which is the major cell membrane antigen of human NK cells, and NK cell activity or cytotoxicity, and demonstrated that it is possible to evaluate NK cell function using the CD56 antigen as an index. This simple evaluation method is useful for functional evaluation of NK cells and the search for activators. However, it requires blood sampling and preparation of NK cells because it uses human NK cells. Therefore, in this study, we examined whether the CD56 antigen functions as an activation index using KHYG‐1 human NK-like cultured cells as a substitute for NK cells.
As a result, the CD56antigen on the KHYG‐1cell membrane was increased in a concentration-dependent manner by IL‐2 stimulation, as was the cytotoxicity. This suggests that the CD56 antigen on the KHYG‐1 cell membrane can be used as an evaluation index of NK activity as in human NK cells.
Journal Title
Shikoku Acta Medica
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
75
Issue
5-6
Start Page
165
End Page
170
Sort Key
165
Published Date
2019-12-25
EDB ID
FullText File
language
jpn
TextVersion
Publisher
departments
Medical Sciences
Pharmaceutical Sciences