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ID 114636
タイトル別表記
Evaluation of NK cell function using human NK-like cultured cell, KHYG-1
CD56抗原を指標としたNK様培養細胞の活性評価
著者
佐藤, 瑞樹 誠馨会千葉メディカルセンター
曽根, 淳美 徳島大学
キーワード
NK cell
KHYG‐1
CD56 antigen
IL‐2
資料タイプ
学術雑誌論文
抄録
NK cells play an important role in the elimination of viral infection and tumors. In recent years, immune cell therapy using activated NK cells has attracted attention, and the search for activators and evaluation of NK cell activity have become important. We previously reported a positive correlation between CD56antigen expression, which is the major cell membrane antigen of human NK cells, and NK cell activity or cytotoxicity, and demonstrated that it is possible to evaluate NK cell function using the CD56 antigen as an index. This simple evaluation method is useful for functional evaluation of NK cells and the search for activators. However, it requires blood sampling and preparation of NK cells because it uses human NK cells. Therefore, in this study, we examined whether the CD56 antigen functions as an activation index using KHYG‐1 human NK-like cultured cells as a substitute for NK cells.
As a result, the CD56antigen on the KHYG‐1cell membrane was increased in a concentration-dependent manner by IL‐2 stimulation, as was the cytotoxicity. This suggests that the CD56 antigen on the KHYG‐1 cell membrane can be used as an evaluation index of NK activity as in human NK cells.
掲載誌名
四国医学雑誌
ISSN
00373699
cat書誌ID
AN00102041
出版者
徳島医学会
75
5-6
開始ページ
165
終了ページ
170
並び順
165
発行日
2019-12-25
EDB ID
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
医学系
薬学系