| ID | 29078 |
| Author |
Dainichi, Teruki
Department of Parasitology and Immunology, The University of Tokushima School of Medicine
Maekawa, Yoichi
Department of Parasitology and Immunology, The University of Tokushima School of Medicine
Ishii, Kazunari
Department of Parasitology and Immunology, The University of Tokushima School of Medicine
Himeno, Kunisuke
Department of Parasitology and Immunology, The University of Tokushima School of Medicine
|
| Keywords | Cystatin
Nippostrongylus brasiliensis
cloning
|
| Content Type |
Journal Article
|
| Description | A novel member of the cystatin family, nippocystatin (NbCys), was identified from excretory-secretory (ES)-products of a nematode Nippostrongylus brasiliensis, and the cDNA was cloned and sequenced. The mRNA of NbCys was confirmed to be expressed in both larvae and adults of the parasite. NbCys was translated as a proform with a single domain for secretion and was detected as a 14-kDa mature form in ES-products of the adult worm. Recombinant protein of NbCys profoundly inhibited the activity of cysteine proteases such as cathepsin L and B, but not that of cathepsin D, an aspartic protease. Furthermore, the ES-products had also been confirmed to inhibit cysteine proteases. Taken together, NbCys may play a role in evasion of N. brasiliensis from host defense systems, since cysteine proteases are known to participate in immune systems of infected hosts.
|
| Journal Title |
The journal of medical investigation : JMI
|
| ISSN | 13431420
|
| NCID | AA11166929
|
| Volume | 48
|
| Issue | 1-2
|
| Start Page | 81
|
| End Page | 87
|
| Sort Key | 81
|
| Published Date | 2001
|
| Remark | |
| EDB ID | |
| FullText File | |
| language |
eng
|
