| ID | 29078 |
| 著者 |
大日, 輝記
Department of Parasitology and Immunology, The University of Tokushima School of Medicine
マエカワ, ヨウイチ
Department of Parasitology and Immunology, The University of Tokushima School of Medicine
イシイ, カズナリ
Department of Parasitology and Immunology, The University of Tokushima School of Medicine
ヒメノ, クニスケ
Department of Parasitology and Immunology, The University of Tokushima School of Medicine
|
| キーワード | Cystatin
Nippostrongylus brasiliensis
cloning
|
| 資料タイプ |
学術雑誌論文
|
| 抄録 | A novel member of the cystatin family, nippocystatin (NbCys), was identified from excretory-secretory (ES)-products of a nematode Nippostrongylus brasiliensis, and the cDNA was cloned and sequenced. The mRNA of NbCys was confirmed to be expressed in both larvae and adults of the parasite. NbCys was translated as a proform with a single domain for secretion and was detected as a 14-kDa mature form in ES-products of the adult worm. Recombinant protein of NbCys profoundly inhibited the activity of cysteine proteases such as cathepsin L and B, but not that of cathepsin D, an aspartic protease. Furthermore, the ES-products had also been confirmed to inhibit cysteine proteases. Taken together, NbCys may play a role in evasion of N. brasiliensis from host defense systems, since cysteine proteases are known to participate in immune systems of infected hosts.
|
| 掲載誌名 |
The journal of medical investigation : JMI
|
| ISSN | 13431420
|
| cat書誌ID | AA11166929
|
| 巻 | 48
|
| 号 | 1-2
|
| 開始ページ | 81
|
| 終了ページ | 87
|
| 並び順 | 81
|
| 発行日 | 2001
|
| 備考 | |
| EDB ID | |
| フルテキストファイル | |
| 言語 |
eng
|
