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ID 114900
Author
Kameda, Tetsuro Kyoto University
Kimura, Yu Kyoto University
Imai, Hirohiko Kyoto University
Matsuda, Tetsuya Kyoto University
Sando, Shinsuke The University of Tokyo
Toshimitsu, Akio Kyoto University
Aoyama, Yasuhiro Kyoto University
Kondo, Teruyuki Kyoto University
Keywords
dopamine
L-dopa
metabolic analysis
neurotransmitters
stable isotope enrichment
triple-resonance NMR
Content Type
Journal Article
Description
In an attempt to monitor μM-level trace constituents, we applied here 1H-{13C-15N} triple-resonance nuclear magnetic resonance (NMR) to 13C/15N-enriched L-Dopa as the inevitable precursor of the neurotransmitter dopamine in the brain. The perfect selectivity (to render endogenous components silent) and μM-level sensitivity (700 MHz spectrometer equipped with a cryogenic probe) of triple-resonance allowed the unambiguous and quantitative metabolic and pharmacokinetic analyses of administered L-Dopa/dopamine in the brain and liver of mice. The level of dopamine generated in the brain (within the range 7–76 μM, which covers the typical stimulated level of ~30 μM) could be clearly monitored ex vivo, but was slightly short of the detection limit of a 7T MR machine for small animals. This work suggests that μM-level trace constituents are potential targets of ex vivo monitoring as long as they contain N atom(s) and their appropriate 13C/15N-enrichment is synthetically accessible.
Journal Title
ChemistryOpen
ISSN
21911363
Publisher
Wiley-VCH Verlag GmbH & Co. KGaA
Volume
5
Issue
2
Start Page
125
End Page
128
Published Date
2015-12-30
Rights
© 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License(https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry