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ID 117203
Title Alternative
Monitoring of Bacterial-contamination of Dental Unit Water Lines using ATP-bioluminescence
Bacterial-contamination Monitoring
Author
Yokota, Kenji Okayama University
Kokeguchi, Susumu Okayama University
Keywords
ATP bioluminescence
Dental unit water line
Bacteria
Contamination
Infection control
ATP-bioluminescence analysis
Bacteria contamination
Content Type
Journal Article
Description
Bacterial contamination of dental unit waterlines (DUWLs) was evaluated by using ATP-bioluminescence analysis and conventional culture method. Water samples (n=44) from DUWLs were investigated for heterotrophic bacteria by culture on R2A agar, which ranged from 1.4×103 to 2.7×105 CFU/mL. The ATP-bioluminescence results for DUWL samples were ranged from 6 to 1189 RLU and obtained within one minutes. These results were well correlated with the culture results (r=0.727-0.855). We conclude that differences in the bacterial contamination of each water supply were confirmed by the ATP-bioluminescence assay. This method would be potentially useful for rapid and simple monitoring of DUWL bacterial contamination.
Journal Title
Journal of Hospital Infection
ISSN
01956701
NCID
AA00258209
AA11538375
Publisher
The Healthcare Infection Society|Elsevier
Volume
94
Issue
4
Start Page
393
End Page
396
Published Date
2016-08-06
Rights
© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Oral Sciences