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ID 118518
Title Alternative
P. gingivalis OMVs induce neuroinflammation
Yoshida, Kayo Tokushima University
Seyama, Mariko Tokushima University
Mekata, Mana Tokushima University
cerebral ventricle
outer membrane vesicle
Porphyromonas gingivalis
Content Type
Journal Article
Objective: Porphyromonas gingivalis (Pg) is thought to be involved in the progression of Alzheimer's disease (AD). Whether Pg or its contents can reach the brain and directly affect neuropathology is, however, unknown. Here, we investigated whether outer membrane vesicles (OMVs) of Pg translocate to the brain and induce the pathogenic features of AD.
Material and Methods: Pg OMVs were injected into the abdominal cavity of mice for 12 weeks. Pg OMV translocation to the brain was detected by immunohistochemistry using an anti-gingipain antibody. Tau protein and microglial activation in the mouse brain were examined by western blotting and immunohistochemistry. The effect of gingipains on inflammation was assessed by real-time polymerase chain reaction using human microglial HMC3 cells.
Results: Gingipains were detected in the region around cerebral ventricles, choroid plexus, and ventricular ependymal cells in Pg OMV-administered mice. Tau and phosphorylated Tau protein increased and microglia were activated. Pg OMVs also increased the gene expression of proinflammatory cytokines in HMC3 cells in a gingipain-dependent manner.
Conclusion: Pg OMVs, including gingipains, can reach the cerebral ventricle and induce neuroinflammation by activating microglia. Pg OMVs may provide a better understanding of the implications of periodontal diseases in neurodegenerative conditions such as AD.
Journal Title
Oral Diseases
Wiley Periodicals
Published Date
This is the peer reviewed version of the following article: Yoshida, K., Yoshida, K., Seyama, M., Hiroshima, Y., Mekata, M., Fujiwara, N., Kudo, Y. and Ozaki, K., Porphyromonas gingivalis outer membrane vesicles in cerebral ventricles activate microglia in mice. Oral Diseases, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
DOI (Published Version)
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Oral Sciences
Institute of Advanced Medical Sciences