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ID 118518
タイトル別表記
P. gingivalis OMVs induce neuroinflammation
著者
Yoshida, Kayo Tokushima University
瀬山, 真莉子 Tokushima University
Mekata, Mana Tokushima University
キーワード
cerebral ventricle
gingipain
microglia
neuroinflammation
outer membrane vesicle
Porphyromonas gingivalis
資料タイプ
学術雑誌論文
抄録
Objective: Porphyromonas gingivalis (Pg) is thought to be involved in the progression of Alzheimer's disease (AD). Whether Pg or its contents can reach the brain and directly affect neuropathology is, however, unknown. Here, we investigated whether outer membrane vesicles (OMVs) of Pg translocate to the brain and induce the pathogenic features of AD.
Material and Methods: Pg OMVs were injected into the abdominal cavity of mice for 12 weeks. Pg OMV translocation to the brain was detected by immunohistochemistry using an anti-gingipain antibody. Tau protein and microglial activation in the mouse brain were examined by western blotting and immunohistochemistry. The effect of gingipains on inflammation was assessed by real-time polymerase chain reaction using human microglial HMC3 cells.
Results: Gingipains were detected in the region around cerebral ventricles, choroid plexus, and ventricular ependymal cells in Pg OMV-administered mice. Tau and phosphorylated Tau protein increased and microglia were activated. Pg OMVs also increased the gene expression of proinflammatory cytokines in HMC3 cells in a gingipain-dependent manner.
Conclusion: Pg OMVs, including gingipains, can reach the cerebral ventricle and induce neuroinflammation by activating microglia. Pg OMVs may provide a better understanding of the implications of periodontal diseases in neurodegenerative conditions such as AD.
掲載誌名
Oral Diseases
ISSN
16010825
cat書誌ID
AA11631042
AA11044105
出版者
Wiley Periodicals
発行日
2022-10-20
権利情報
This is the peer reviewed version of the following article: Yoshida, K., Yoshida, K., Seyama, M., Hiroshima, Y., Mekata, M., Fujiwara, N., Kudo, Y. and Ozaki, K., Porphyromonas gingivalis outer membrane vesicles in cerebral ventricles activate microglia in mice. Oral Diseases, which has been published in final form at https://doi.org/10.1111/odi.14413. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
odi.pdf 1.47 MB
言語
eng
著者版フラグ
著者版
部局
歯学系
先端酵素学研究所