Tanegashima, Kosuke Tokyo Metropolitan Institute of Medical Science
Takahashi, Rena Tokyo Metropolitan Institute of Medical Science|Tokyo Medical and Dental University
Nuriya, Hideko Tokyo Metropolitan Institute of Medical Science
Iwase, Rina Tokyo Metropolitan Institute of Medical Science|Chuo University
Naruse, Naoto Tokushima University
Tsuji, Kohei Tokushima University
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Hara, Takahiko Tokyo Metropolitan Institute of Medical Science|Tokyo Medical and Dental University
CXCL14 is a primordial chemokine that plays multiple roles in tumor suppression, autoimmune arthritis, and obesity-associated insulin resistance. However, the underlying molecular mechanisms are unclear. Here, we show that CXCL14 transports various types of CpG oligodeoxynucleotide (ODN) into the endosomes and lysosomes of bone marrow-derived dendritic cells (DCs), thereby activating Toll-like receptor 9 (TLR9). A combination of CpG ODN (ODN2395) plus CXCL14 induced robust production of IL-12 p40 by wild-type, but not Tlr9-knockout, DCs. Consistent with this, ODN2395-mediated activation of DCs was significantly attenuated in Cxcl14-knockout mice. CXCL14 bound CpG ODN with high affinity at pH 7.5, but not at pH 6.0, thereby enabling efficient delivery of CpG ODN to TLR9 in the endosome/lysosome. Furthermore, the CXCL14-CpG ODN complex specifically bound to high affinity CXCL14 receptors on DCs. Thus, CXCL14 serves as a specific carrier of CpG DNA to sensitize TLR9-mediated immunosurveillance.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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ebiom_24_247.pdf 1.49 MB