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ID 109693
Title Alternative
5-HT2A/2C受容体アゴニストである1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropaneはセロトニン神経に対するエタノールの神経発生毒性を軽減する
DOI mitigates DNT of EtOH to 5-HT cells
Author
Ishiguro, Tsukasa Tokushima University
Keywords
1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane
Fetal ethanol
Rat
5-HTergic neuron
Midbrain raphe nuclei
Content Type
Thesis or Dissertation
Description
Prenatal ethanol exposure causes the reduction of serotonergic (5-HTergic) neurons in the midbrain raphe nuclei. In the present study, we examined whether an activation of signaling via 5-HT2A and 5-HT2C receptors during the fetal period is able to prevent the reduction of 5-HTergic neurons induced by prenatal ethanol exposure. Pregnant Sprague-Dawley rats were given a liquid diet containing 2.5 to 5.0% (w/v) ethanol on gestational days (GDs) 10 to 20 (Et). As a pair-fed control, other pregnant rats were fed the same liquid diet except that the ethanol was replaced by isocaloric sucrose (Pf). Each Et and Pf group was subdivided into two groups; one of the groups was treated with 1 mg/kg (i.p.) of 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI), an agonist for 5-HT2A/2C receptors, during GDs 13 to 19 (Et-DOI or Pf-DOI), and another was injected with saline vehicle only (Et-Sal or Pf-Sal). Their fetuses were removed by cesarean section on GD 19 or 20, and fetal brains were collected. An immunohistological examination of 5-HTergic neurons in the fetuses on embryonic day 20 using an antibody against tryptophan hydroxylase revealed that the number of 5-HTergic neurons in the midbrain raphe nuclei was significantly reduced in the Et-Sal fetuses compared to that of the Pf-Sal and Pf-DOI fetuses, whereas there were no significant differences between Et-DOI and each Pf control. Thus, we concluded that the reduction of 5-HTergic neurons that resulted in prenatal ethanol exposure could be alleviated by the enhancement of signaling via 5-HT2A/2C receptors during the fetal period.
Journal Title
Congenital Anomalies
ISSN
17414520
Publisher
Japanese Teratology Society|Wiley
Volume
56
Issue
4
Start Page
163
End Page
171
Published Date
2015-12-30
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201606071014.pdf
論文本文 : k2954_fulltext.pdf
著者の申請により要約(2016-06-07公開)に替えて論文全文を公開(2018-06-14)
本論文は, 著者Tsukasa Ishiguroの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2954号
Diploma Number
甲医第1287号
Granted Date
2016-03-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences