Vascular endothelial growth factors and their receptors in the novel human cell line, HN-Eso-1, established from esophageal spindle cell carcinoma

Human carcinosarcomas of esophagus are uncommon malignant neoplasms that
are composed both carcinomatous and sarcomatous components. We established a novel
cell line, HN-Eso-1, from the metastatic esophageal spindle cell carcinoma (so-called carcinosarcoma).
In this study, we estimated the vascular endothelial growth factors (VEGFs)
and VEGF receptors (VEGFRs). Reverse transcription polymerase chain reaction (RTPCR)
studies revealed that VEGF-A,-C,-D and VEGFR-1,-2 were upregulated. Cisplatin
reduced the cell viability of HN-Eso-1 cells and VEGF attenuated its effect. These results
suggest that expression of VEGF-A, VEGF-C, VEGF-D, VEGFR-1, and VEGFR-2 are involved
in the cell’s autocrine system and that VEGF protected these cells from the antitumor
agent.

The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html