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ID 114840
Title Alternative
ニッケルアレルギー惹起相におけるセマフォリン7Aの効果
Author
Minami, Norikazu Tokushima University
Lipei, Liu Tokushima University
Yunizar, Mohammad Fadyl Tokushima University
Keywords
Nickel Allergy
Semaphorin7A
Keratinocyte
Content Type
Thesis or Dissertation
Description
Background: Metal allergy is caused by many factors, including cells, cytokines, chemokines, or the environment. Recent studies suggested semaphorin7A (Sema7A), expressed on activated T cells, is crucial to produce inflammation through α1β1 integrin on monocytes and macrophages. However, the role of Sema7A on keratinocytes in metal allergy is still unclear. In this study, we focused on keratinocytes since they are known as an important player for skin immunity, and analyzed the effect of Sema7A expressed on keratinocytes in the development of metal allergy.
Materials and Methods: Mouse keratinocyte line PAM2.12 cells were treated with NiCl2 to analyze the expression of Sema7A. Ni allergy was induced in female C57BL/6J mice (6-8 weeks old) with or without Sema7A suppression to confirm if Sema7A is necessary to produce allergic reactions to NiCl2.
Results: NiCl2 enhanced the expression of Sema7A in a dose and time-dependent manner after 72 hours of stimulation. PAM 2.12 produced TNF-α in response to NiCl2, and this secretion was reduced by Sema7A inhibition. In a mouse model, ear thickness, at 48 hours after NiCl2 injection, was significantly decreased by Sema7A siRNA administration.
Conclusions: Sema7A is essential to produce an allergic reaction to NiCl2, especially during the effector phase. Since the interaction between Sema7A and α1β1 integrin enhances inflammation in many skin diseases, this interaction may also have possibilities to be a therapeutic target for metal allergy.
Journal Title
Journal of Oral Health and Biosciences
ISSN
21896682
Publisher
Shikoku Society of Dental Research
Volume
33
Issue
1
Start Page
8
End Page
14
Sort Key
8
Published Date
2020-06-12
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Norikazu MINAMIの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3427号
Diploma Number
甲口第463号
Granted Date
2020-05-14
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
University Hospital
Oral Sciences