number of access : ?
number of downloads : ?
ID 109407
Title Alternative
遠赤外線エネルギーを放射するセラミックス(流紋岩)は骨形成を促進する
FIR Ceramics Promote the Formation of Bone
Author
Dolgorsuren, Aldartsogt The University of Tokushima
Yamashita, Kikuji The University of Tokushima KAKEN Search Researchers
Dalkhsuren, Shine-Od University of Mongolia
Seki, Shinichiro The University of Tokushima KAKEN Search Researchers
Keywords
Bone formation
Far-infrared ray
Osteoblast
Content Type
Thesis or Dissertation
Description
Far infrared ray (FIR) energy radiated by the natural ceramics (Rhyolite) cooled FIR ceramics activates water molecules and blood circulation to stimulate skin and other tissues. The aim of our study is to make clear whether the FIR ceramics radiating FIR energy affect or not on the new bone formation in vivo and in vitro. Methods: MC3T3-E1 cells were cultured in FIR CO2 incubator. The cell proliferation and the gene expression were analyzed by using WST-8 assay kit, RT-PCR and micro array analysis. The enzyme activities were analyzed by using the apiRZYM kit. Furthermore, titanium and natural FIR ceramics compounds were implanted under the periosteium of rat skull bone by injection method. Four weeks later, the samples were examined by the light microscope and micro CT analyses. Results: Proliferation of MC3T3-E1 cell was and DNA concentrations were inhibited by FIR energy radiation. The ALP activities were accelerated and the area of calcification nodules increased on 4 weeks. The RT-PCR data showed that the gene Runx2, Osterix, BSP, OCN, Col1a1 and OPN expression of MC3T3-E1 osteoblast like cells was activated. Bone mineral density (BMD mg/cm²) of implanted sites of T50-F50, T25-F75 and F100 groups was significantly enhanced after 4 weeks compared with control groups. This data shows FIR energy radiation by the natural FIR ceramics promoted bone-forming activity of osteoblasts. Significance: This study suggested that new bioactive ceramics such us natural FIR ceramics was useful for some clinically applications to repair bone defects for example of dental implant surgery.
Journal Title
Journal of Hard Tissue Biology
ISSN
1880828X
13417649
NCID
AA11074332
AA11653227
Publisher
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
Volume
23
Issue
4
Start Page
423
End Page
434
Published Date
2014-10-21
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201506091002.pdf
審査要旨 : LID201506091003.pdf
論文本文 : LID201506091004.pdf
本論文は, 著者Aldartsogt Dolgorsurenの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
© 2014 The Hard Tissue Biology Network Association
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2808号
Diploma Number
甲口第390号
Granted Date
2015-03-23
Degree Name
Doctor of Philosophy
Grantor
Tokushima University
departments
Oral Sciences