アクセス数 : ?
ダウンロード数 : ?
ID 109407
タイトル別表記
遠赤外線エネルギーを放射するセラミックス(流紋岩)は骨形成を促進する
FIR Ceramics Promote the Formation of Bone
著者
ドルゴルスレン, アルダルツォグト 徳島大学大学院口腔科学教育部(口腔科学専攻)
山下, 菊治 The University of Tokushima KAKEN研究者をさがす
Dalkhsuren, Shine-Od University of Mongolia
関, 伸一郎 The University of Tokushima KAKEN研究者をさがす
キーワード
Bone formation
Far-infrared ray
Osteoblast
資料タイプ
学位論文
抄録
Far infrared ray (FIR) energy radiated by the natural ceramics (Rhyolite) cooled FIR ceramics activates water molecules and blood circulation to stimulate skin and other tissues. The aim of our study is to make clear whether the FIR ceramics radiating FIR energy affect or not on the new bone formation in vivo and in vitro. Methods: MC3T3-E1 cells were cultured in FIR CO2 incubator. The cell proliferation and the gene expression were analyzed by using WST-8 assay kit, RT-PCR and micro array analysis. The enzyme activities were analyzed by using the apiRZYM kit. Furthermore, titanium and natural FIR ceramics compounds were implanted under the periosteium of rat skull bone by injection method. Four weeks later, the samples were examined by the light microscope and micro CT analyses. Results: Proliferation of MC3T3-E1 cell was and DNA concentrations were inhibited by FIR energy radiation. The ALP activities were accelerated and the area of calcification nodules increased on 4 weeks. The RT-PCR data showed that the gene Runx2, Osterix, BSP, OCN, Col1a1 and OPN expression of MC3T3-E1 osteoblast like cells was activated. Bone mineral density (BMD mg/cm²) of implanted sites of T50-F50, T25-F75 and F100 groups was significantly enhanced after 4 weeks compared with control groups. This data shows FIR energy radiation by the natural FIR ceramics promoted bone-forming activity of osteoblasts. Significance: This study suggested that new bioactive ceramics such us natural FIR ceramics was useful for some clinically applications to repair bone defects for example of dental implant surgery.
掲載誌名
Journal of Hard Tissue Biology
ISSN
1880828X
13417649
cat書誌ID
AA11074332
AA11653227
出版者
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
23
4
開始ページ
423
終了ページ
434
発行日
2014-10-21
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201506091002.pdf
審査要旨 : LID201506091003.pdf
論文本文 : LID201506091004.pdf
本論文は, 著者Aldartsogt Dolgorsurenの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
© 2014 The Hard Tissue Biology Network Association
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2808号
学位記番号
甲口第390号
学位授与年月日
2015-03-23
学位名
博士(学術)
学位授与機関
徳島大学
部局
歯学系