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ID 105888
タイトル別表記
脂肪由来幹細胞の栄養因子を介した肝傷害に対しての保護効果
著者
齋藤, 裕 徳島大学大学院医科学教育部(医学専攻) 徳島大学 教育研究者総覧 KAKEN研究者をさがす
宇都宮, 徹 The University of Tokushima KAKEN研究者をさがす
Sugimoto, Kouji The University of Tokushima
Asanoma, Michihito The University of Tokushima
キーワード
Adipose derived stem cells
Hepatocytes
Vascular endothelial growth factor
資料タイプ
学位論文
抄録
Background
In this study we investigated whether adipose-derived stem cells (ADSCs) had any beneficial protective effects on liver injury and regeneration in vivo. Moreover, we examined whether ADSCs protect hepatocytes by trophic molecules.
Materials and Methods
We transplanted ADSCs into mice after 70% hepatectomy (Hx) and ischemia-reperfusion (I/R), and observed liver injury and regeneration after reperfusion. We co-cultured hepatocytes with ADSCs using a Transwell System for seven days and evaluated the viabilities of hepatocytes and the cytokine levels in the culture medium. Bevacizumab was used in order to confirm the effect of VEGF on hepatocytes.
Results
ADSCs improved serum liver function at six hours after reperfusion in a non-lethal model and stimulated liver regeneration at 24 hours after reperfusion in a lethal model. VEGF levels in the culture medium increased by co-culture ADSCs with hepatocytes. ADSCs improved the viabilities of hepatocytes. The inhibited production of VEGF by bevacizumab did not affect the viability of hepatocytes.
Conclusions
ADSCs were able to ameliorate liver injury and stimulate liver regeneration in subsequent Hx and I/R injured model mice. Furthermore, hepatocytes were protected by the trophic molecules of the ADSCs. However, such protective effects might be provided by mechanisms other than VEGF signaling.
掲載誌名
Journal of Surgical Research
ISSN
00224804
cat書誌ID
AA00708021
出版者
Elsevier
180
1
開始ページ
162
終了ページ
168
発行日
2012-10-25
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201308190003.pdf
論文本文 : LID201405260001.pdf
本論文は, 著者Yu Saitoの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2618号
学位記番号
甲医第1163号
学位授与年月日
2013-05-23
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院