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ID 105960
著者
Sun, Yuanzhi Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
Enkhjargal, Molomjamts Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
スギハラ, アヤ Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
ヤマダ, サキ Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
Chen, Xiaohui Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
ミウラ, ユカリ Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
フクナガ, エリ Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
佐藤, 征弥 Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
小山, 保夫 Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima KAKEN研究者をさがす
キーワード
triclocarban
thymocytes
membrane potential
flow cytometer
資料タイプ
紀要論文
抄録
The effect of triclocarban (TCC), an environmental pollutant from household items and health care products, on membrane potential of rat thymocytes was examined by a flow cytometry with a fluorescent probe sensitive to membrane potential, bis-(1,3-dibutylbarbituric acid)trimethine oxonol, because TCC changes intracellular ionic circumstance that may affect the membrane potential. TCC at 0.3 μM or more (up to 3 μM) depolarized the membranes. This TCC-induced phenomenon was against our prediction because TCC increases intracellular Ca2+ concentration that activates Ca2+-dependent K+ channels, resulting in a hyperpolarization. The depolarization was still observed under Ca2+-free condition, but not under Na+-free condition. Furthermore, TCC hyperpolarized the membranes under Ca2+- and Na+-free condition. To see if TCC inhibits Ca2+-dependent hyperpolarization, the effect of A23187, a calcium ionophore, on the membrane potential was examined in the cells treated with TCC. A23187 induced large depolarization in the cells treated with 0.3–3 μM TCC. The A23187-induced depolarization in the presence of TCC was greatly attenuated under Na+-free or Ca2+-free condition whereas A23187 elicited hyperpolarization in the cells treated with 0.3–3 μM TCC under Ca2+- and Na+-free condition. Results suggest that 0.3–3 μM TCC increases membrane permeability of Na+ and Ca2+, resulting in the depolarization. Large depolarization induced by TCC in the presence of external Ca2+ and Na+ may mask the hyperpolarization elicited via the increase in intracellular Ca2+ concentration by TCC. Thus, there is a possibility that TCC depolarizes membranes of lymphocytes, resulting in alteration of cellular functions of lymphocytes.
掲載誌名
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
cat書誌ID
AN10065859
27
4
開始ページ
53
終了ページ
59
並び順
53
発行日
2013-10
備考
P.59のページ付に誤植(P.57と表記)
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系