ID | 106279 |
タイトル別表記 | Streptococcus intermedius バイオフィルム形成に及ぼす細胞外DNA及びDNA結合タンパク質の影響
Effect of eDNA on biofilm formation
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著者 |
アシキン, ヌル
徳島大学大学院口腔科学教育部(口腔科学専攻)
Liu, Dali
Shanghai Jiao Tong University
Shu, Rong
Shanghai Jiao Tong University
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キーワード | extracellular DNA
extracellular HLP
biofilm development
Streptococcus intermedius
DNA concentration
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資料タイプ |
学位論文
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抄録 | Aims: The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity.
Methods and Results: Formed biofilm mass was measured with 0.1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml-1) markedly decreased biofilm formation and cell density in biofilms. Co-localization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml-1) purified from S. intermedius, other Gram-positive, –negative bacteria, or human KB cells into the S. intermedius culture increased the biofilm mass of all tested strains of S. intermedius, wild-type, HLP down-regulated strain, and control strains. In contrast, the addition of eDNA (> 1 μg ml-1) decreased the biofilm mass of all S. intermedius strains. Conclusions: These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity. Significance and Impact of the Study: eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases. |
掲載誌名 |
Journal of Applied Microbiology
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ISSN | 13652672
13645072
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cat書誌ID | AA11115564
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出版者 | The Society for Applied Microbiology|John Wiley & Sons Inc
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巻 | 115
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号 | 1
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開始ページ | 260
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終了ページ | 270
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発行日 | 2013-03-29
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備考 | 内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201409191007.pdf 審査要旨 : LID201409191008.pdf 論文本文 : k2757_fulltext.pdf 本論文は,著者Asikin Nurの学位論文として提出され,学位審査・授与の対象となっている。 著者の申請により要約(2014-09-20公開)に替えて論文全文を公開(2019-10-09) |
権利情報 | This is the peer reviewed version of the following article: Nur, A., Hirota, K., Yumoto, H., Hirao, K., Liu, D., Takahashi, K., Murakami, K., Matsuo, T., Shu, R. and Miyake, Y. (2013), Effects of extracellular DNA and DNA‐binding protein on the development of a Streptococcus intermedius biofilm. J Appl Microbiol, 115: 260-270., which has been published in final form at https://doi.org/10.1111/jam.12202. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第2757号
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学位記番号 | 甲口第386号
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学位授与年月日 | 2014-09-10
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学位名 |
博士(歯学)
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学位授与機関 |
徳島大学
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部局 |
歯学系
病院
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