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ID 109378
タイトル別表記
ラット腎不全初期における心臓リモデリングには心筋タンパク質と脂質の増加が関与している
著者
桑原, 三恵子 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Bannai, Kenji Kureha Corporation
Yamato, Hideyuki Kureha Corporation
キーワード
Cardiac remodeling
Chronic kidney disease
FTIR spectroscopy
Uremic toxin
Lipid accumulation
資料タイプ
学位論文
抄録
Chronic kidney disease (CKD) is associated with increased risks of cardiovascular morbidity and mortality. Cardiac remodeling including myocardial fibrosis and hypertrophy is frequently observed in CKD patients. In this study, we investigate the mechanism involved in cardiac hypertrophy associated with CKD using a rat model, by morphological and chemical component changes of the hypertrophic and non-hypertrophic hearts. Sprague–Dawley rats were 4/5 nephrectomized (Nx) at 11 weeks of age and assigned to no treatment and treatment with AST-120,which was reported to affect the cardiac damage, at 18 weeks of age. At 26 weeks of age, the rats were euthanized under anesthesia, and biochemical tests as well as analysis of cardiac condition were performed by histological and spectrophotometric methods. Cardiac hypertrophy and CKD were observed in 4/5 Nx rats even though vascular calcification and myocardial fibrosis were not detected. The increasing myocardial protein was confirmed in hypertrophic hearts by infrared spectroscopy. The absorption of amide I and other protein bands in hypertrophic hearts increased at the same position as in normal cardiac absorption. Infrared spectra also showed that lipid accumulation was also detected in hypertrophic heart. Conversely, the absorptions of protein were obviously reduced in the myocardium of non-hypertrophic heart with CKD compared to that of hypertrophic heart. The lipid associated absorption was also decreased in non-hypertrophic heart. Our results suggest that cardiac remodeling associated with relatively early-stage CKD may be suppressed by reducing increased myocardial protein and ameliorating cardiac lipid load.
掲載誌名
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
ISSN
09254439
cat書誌ID
AA10779707
AA11522998
出版者
Elsevier
1842
9
開始ページ
1433
終了ページ
1443
発行日
2014-05-02
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201505251021.pdf
論文本文 : k2848_fulltext.pdf
本論文は,著者Mieko Kuwaharaの学位論文として提出され, 学位審査・授与の対象となっている。
著者の申請により要約(2015-05-26公開)に替えて論文全文を公開(2020-03-18)
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2848号
学位記番号
甲栄第226号
学位授与年月日
2015-03-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系