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ID 109479
タイトル別表記
MC3T3-E1細胞における圧縮応力誘導性miR494-3pによる細胞増殖の阻害
FUNCTION OF miR-494-3p IN OSTEOBLASTS
著者
岩脇, 有軌 徳島大学大学院口腔科学教育部(口腔科学専攻) 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Higaki, Nobuaki Tokushima University
キーワード
Mechanical stimuli
Compressive force
MicroRNA
Osteoblasts
Cell proliferation
資料タイプ
学位論文
抄録
Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3'-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation in osteoblasts by up-regulating miR-494-3p followed by FGFR2 and ROCK1 gene repressions.
掲載誌名
Journal of Bioscience and Bioengineering
ISSN
13474421
13891723
cat書誌ID
AA11644703
AA11307678
出版者
The Society for Biotechnology, Japan|Elsevier
120
4
開始ページ
456
終了ページ
462
発行日
2015-03-17
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201507171004.pdf
審査要旨 : LID201507171005.pdf
論文本文 : k2858_fulltext.pdf
本論文は,著者Yuki Iwawakiの学位論文として提出され,学位審査・授与の対象となっている。
著者の申請により要約(2015-07-18公開)に替えて論文全文を公開(2019-08-22)
権利情報
Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
This manuscript version is made available under the CC-BY-NC-ND 4.0 license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2858号
学位記番号
甲口第397号
学位授与年月日
2015-06-11
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系
病院