ID 109541
著者
堀口, 大吾 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
フカ, ミユキ Department of Applied Molecular Bioscience, Yamaguchi University
フジサワ, コウイチ Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School|Center for Reparative Medicine, Yamaguchi University School of Medicine
谷村, 綾子 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
三好, 圭子 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
ムラカミ, リュウタロウ Department of Applied Molecular Bioscience, Yamaguchi University
野間, 隆文 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
Dak2
gene expression
in silico analysis
microarray
mitochondria
資料タイプ
学術雑誌論文
抄録
Adenylate kinase isozyme 2 (AK2) is located in mitochondrial intermembrane
space and regulates energy metabolism by reversibly converting ATP and AMP to 2 ADPs.
We previously demonstrated that disruption of the Drosophila melanogaster AK2 gene
(Dak2) resulted in growth arrest during the larval stage and subsequent death. Two other
groups found that human AK2 mutations cause reticular dysgenesis, a form of severe combined
immunodeficiency (SCID) that is associated with severe hematopoietic defects and
sensorineural deafness. However, the mechanisms underlying differential outcomes of AK2
deficiency in Drosophila and human systems remain unknown. In this study, effects of
tissue-specific inactivation of the Dak2 gene on Drosophila development were analyzed
using RNAi-mediated gene knockdown. In addition, to investigate the roles of AK2 in the
regulation of gene expression during development, microarray analysis was performed using
RNA from first and second instar larvae of Dak2-deficient mutant and wild-type D.
melanogaster. Knockdown of Dak2 in all germ layers caused cessation of growth and subsequent
death of flies. Microarray analysis revealed that Dak2 deficiency downregulates
various genes, particularly those involved in the proteasomal function and in mitochondrial
translation machinery. These data indicate that adenine nucleotide interconversion by
Dak2 is crucial for developmental processes of Drosophila melanogaster.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
61
1-2
開始ページ
137
終了ページ
150
並び順
137
発行日
2014-02
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系