アクセス数 : ?
ダウンロード数 : ?
ID 109571
タイトル別表記
プロポフォールによるラット大動脈の弛緩性は年齢により減弱する
Propofol-induced vasodilation and aging
著者
酒井, 陽子 徳島大学大学院医学研究科(外科系専攻) 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キノシタ, ヒロユキ Aichi Medical University
ハタケヤマ, ノボル Aichi Medical University
アズマ, トシハル National Center for Global Health and Medicine
キーワード
propofol
vasorelaxation
rat aorta
aging
endothelium
資料タイプ
学位論文
抄録
Background : Propofol causes vasodilation via endothelium-dependent and -independent mechanisms. Because endothelial function is impaired with aging, the effects of propofol on endothelium-dependent vasodilation might be altered by aging. The aim of this study was thus to determine the effects of aging on vascular responses to propofol. Methods : Young (4-6 weeks old) or adult (16-25 weeks old) rats were anesthetized with sevoflurane. The thoracic aorta was dissected and cut into pieces 3-4mm in length. In some rings, the endothelium was deliberately removed. The ring segment of the aorta was mounted for isometric force recording at a resting tension of 0.5-1.0 g in a 2 ml organ bath, containing Krebs-Ringer bicarbonate buffer. Arteries were precontracted with phenylephrine, and the function of endothelium was confirmed with acetylcholine. Then, we studied the concentration-dependent effects of propofol in endothelium-intact (control group) and -denuded aortic rings (denuded group), as well as those treated with N[ω]-nitro-L-arginine methylester (L-NAME group). Results : Relaxation due to propofol was observed in the control groups of both young and adult rats in a concentration-dependent manner, but the magnitude of relaxation was significantly greater in young rats. In addition, in young rats, relaxation due to propofol was significantly and equally reduced in both L-NAME and denuded groups at all propofol concentrations that we studied (10[-6]-10[-3] M). In adult rats, relaxation due to propofol was quite similar between control and L-NAME groups at all propofol concentrations, whereas it was significantly reduced in the denuded group. Conclusion : These results suggest that endothelium-derived nitric oxide plays an important role in propofol-induced vasodilation in young rats, but not in adult rats.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
61
3-4
開始ページ
278
終了ページ
284
並び順
278
発行日
2014-08
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201505141001.pdf
論文本文 : LID201505141002.pdf
本論文は,著者Yoko Sakaiの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2791号
学位記番号
甲医第1233号
学位授与年月日
2015-02-26
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系
歯学系